MicroRNA-30a suppresses the proliferation, migration and invasion of human renal cell carcinoma cells by directly targeting ADAM9.
MicroRNA-30a suppresses the proliferation, migration and invasion of human renal cell carcinoma cells by directly targeting ADAM9.
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DOI：
10.3892/ol.2018.8999
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发表时间：
2018-09
期刊：
影响因子：
2.9
通讯作者：
Li B
中科院分区：
文献类型：
--
作者：
Jiang L;Liu Y;Ma C;Li B
越来越多的研究报道microRNA（miR）-30 a在多种人类癌症中表达异常，可能参与癌症的发生和发展。然而，其在肾细胞癌（RCC）中的表达和作用尚不清楚。在本研究中，逆转录-定量聚合酶链反应（RT-qPCR）进行定量miR-30 a在RCC组织和细胞系中的表达。采用细胞计数kit-8法、迁移和侵袭实验检测miR-30 a对肾癌细胞增殖、迁移和侵袭的影响。通过荧光素酶报告基因分析、RT-qPCR和Western blotting鉴定miR-30 a的靶基因。结果表明，miR-30 a在肾癌组织和肾癌细胞系中的表达均较相应的癌旁组织和正常肾细胞系明显下调。miR-30 a的重新表达抑制了RCC细胞的增殖、迁移和侵袭。此外，ADAM金属肽酶结构域9（ADAM 9）被验证为miR-30 a的直接靶点。此外，通过小干扰RNA敲低ADAM 9能够模拟RCC细胞中miR-30 a过表达的作用。这些结果表明miR-30 a在肾细胞癌的发生发展中起重要作用，恢复miR-30 a可能成为肾细胞癌治疗的一个潜在策略。
An increasing number of studies reported that microRNA (miR)-30a was dysregulated in several types of human cancer and may contribute to cancer carcinogenesis and progression. However, its expression and roles in renal cell carcinoma (RCC) remain unknown. In the present study, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to quantify miR-30a expression in RCC tissues and cell lines. The cell counting kit-8 assay, migration and invasion assays were used to evaluate the roles of miR-30a on the proliferation, migration and invasion of RCC cells. The target gene of miR-30a was identified by luciferase reporter assays, RT-qPCR and western blotting. The results indicated that miR-30a was downregulated in RCC tissues and cell lines compared with corresponding noncancerous tissues and normal renal cell line, respectively. Re-expression of miR-30a inhibited the proliferation, migration and invasion of RCC cells. Additionally, ADAM metallopeptidase domain 9 (ADAM9) was validated as a direct target of miR-30a. Furthermore, the knockdown of ADAM9 by small interfering RNAs was able to mimic the effects of miR-30a overexpression in RCC cells. These results highlight the important role for miR-30a in the occurrence and development of RCC, and the restoration of miR-30a might be investigated as a potential strategy for treating RCC.