Electrophysiological and pharmacological characterization of perforant path synapses in CA1: mediation by glutamate receptors.
Electrophysiological and pharmacological characterization of perforant path synapses in CA1: mediation by glutamate receptors.
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CA1 穿通路径突触的电生理学和药理学特征：谷氨酸受体的介导。
DOI：
10.1152/jn.1992.68.1.1
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发表时间：
1992
影响因子：
2.5
通讯作者：
Levy,WB
中科院分区：
文献类型：
--
作者：
Colbert,CM;Levy,WB
1.使用成年大鼠海马脑片，我们研究了单突触电位在CA 1区引起的刺激无论是穿通路或Schaffer侧支。切除CA 3区和齿状回防止多突触兴奋CA 1和促进解释的细胞外电位。2.刺激Schaffer侧支和穿通通路在海马CA 1区诱发的群体兴奋性突触后电位（pEPSP）在层电位上有明显的差异，刺激穿通通路在海马CA 1区诱发的pEPSP在短潜伏期内呈负向。CA 1和正向pEPSPs在S.辐射状刺激Schaffer侧支诱发s.辐射状3.药理学操作也区分了CA 1中的两种途径。选择性GABAB激动剂巴氯芬可显著降低刺激Schaffer侧支诱发的pEPSPs的斜率，但对刺激穿支诱发的pEPSPs的斜率无明显影响。谷氨酸受体拮抗剂6，7-二硝基喹喔啉-2，3-二酮（DNQX）和2-氨基-5-膦酰基戊酸（APV）的联合应用可阻断s.刺激穿孔通路会诱发陷窝分子。DNQX和APV的这种应用揭示了正向场电位，其被γ-氨基丁酸（GABA）受体拮抗剂印防己毒素或荷包牡丹碱的浴应用所阻断。5.虽然谷氨酸介导的刺激穿孔路径引起的反应的组成部分显然是兴奋性的，我们从来没有观察到一个群体尖峰。通过刺激穿通神经引起的锥体神经。（250字处删节）
1. With the use of hippocampal slices from adult rats, we studied monosynaptic potentials in CA1 evoked by stimulating either the perforant pathway or the Schaffer collaterals. Excision of region CA3 and the dentate gyrus prevented polysynaptic excitation of CA1 and facilitated interpretation of the extracellular potentials. 2. Laminar profiles distinguished the population excitatory postsynaptic potentials (pEPSPs) in CA1 evoked by stimulating the Schaffer collaterals and the perforant path. Stimulating the perforant path evoked short-latency negative-going pEPSPs in s. lacunosum-moleculare of CA1 and positive-going pEPSPs in s. radiatum. Stimulating the Schaffer collaterals evoked negative-going pEPSPs in s. radiatum. 3. A pharmacological manipulation also distinguished the two pathways in CA1. The selective GABAB agonist baclofen greatly decreased the slope of pEPSPs evoked by stimulating the Schaffer collaterals but did not decrease the slope of pEPSPs evoked by stimulating the perforant path. 4. Combined bath application of the glutamate receptor antagonists 6,7-dinitroquinoxaline-2,3-dione (DNQX) and 2-amino-5-phosphonopentanoic acid (APV) abolished the negative-going pEPSPs in s. lacunosum-moleculare evoked by stimulating the perforant pathway. This application of DNQX and APV revealed a positive-going field potential that was blocked by bath application of the gamma-aminobutyric acid (GABA) receptor antagonists picrotoxin or bicuculline. 5. Although the glutamate-mediated component of the response evoked by stimulating the perforant path was apparently excitatory, we never observed a population spike in s. pyramidal evoked by stimulating the perforant path.(ABSTRACT TRUNCATED AT 250 WORDS)