Substance P-containing ganglion cells become progressively less detectable during retinotectal development in the frog Rana pipiens.
Substance P-containing ganglion cells become progressively less detectable during retinotectal development in the frog Rana pipiens.
复制标题
在青蛙视网膜顶盖发育过程中，含 P 物质的神经节细胞逐渐变得难以检测到。
DOI：
10.1073/pnas.83.15.5736
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发表时间：
1986
影响因子：
11.1
通讯作者：
H. Karten
中科院分区：
文献类型：
--
作者：
R. Kuljiš;H. Karten
对Shumway 25期（S25）和Taylor和Kollros 25期（TKXXV）的尖音蛙（Rana pipiens）胚胎和蝌蚪的视网膜和视盖中的P物质样免疫反应（SPLI）进行了化学分析。视网膜神经节细胞（RGC）胞体的群体显示SPLI。标记的细胞体的数量增加的比例和染色强度之间的S25和TKX和进行性减少到变态结束。在TKXXV时，在RGC层中只能观察到视网膜周边偶尔显示SPLI的细胞，这预示着成人情况，其中SPLI只能在偶尔的RGC中罕见地看到。从S25到TKXVI，视神经轴突显示SPLI的比例逐渐增加，此后逐渐减少，直至幼虫期结束。同时，SPLI首次出现在TKIII和TKV之间的浅顶盖神经节，染色强度逐渐增加，并在一个离散的薄层中，先前显示在成人中含有离视网膜终末。这些观察结果证实了推论，从以前的研究表明存在的肽能RGCs，终止在精确限制的突触位点在顶盖和推测执行不同的功能操作的成人。然而，以前的观察，需要各种实验操作，涉及损伤的视觉系统，以证明神经活性肽样免疫反应性的RGCs，从而允许创伤后表达的异常肽表型，可能不反映正常功能的RGCs的可能性。本研究消除了这个变量，并提供了肽能RGCs存在的进一步证据。
Substance P-like immunoreactivity (SPLI) was immunohistochemically analyzed in the retinae and optic tecta of Rana pipiens embryos and tadpoles between stages 25 of Shumway (S25) and XXV of Taylor and Kollros (TKXXV). A population of retinal ganglion cell (RGC) somata display SPLI. The number of labeled cell bodies increases in proportion and staining intensity between S25 and TKX and progressively decreases toward the end of metamorphosis. At TKXXV, only occasional cells in the periphery of the retina displaying SPLI can be observed in the RGC layer, heralding the adult condition, in which SPLI can only be seen rarely in occasional RGCs. An increasing proportion of optic nerve axons display SPLI from S25 through TKXVI, decreasing progressively thereafter toward the end of the larval period. Concurrently, SPLI appears for the first time in the superficial tectal neuropil between TKIII and TKV, with progressively increasing staining intensity and in a discrete lamina previously shown to contain retinofugal terminals in the adult. These observations corroborate inferences from previous studies indicating the existence of populations of peptidergic RGCs that terminate within precisely restricted synaptic loci in the tectum and presumably perform different functional operations in the adult. Previous observations, however, necessitated various experimental manipulations involving injuries to the visual system in order to demonstrate neuroactive peptide-like immunoreactivity in RGCs, thus allowing the possibility of posttraumatic expression of anomalous peptide phenotypes that may not reflect normal features of RGCs. The present study eliminates this variable and provides further evidence of the existence of peptidergic RGCs.