Differential express-ion of STAT5 and Bcl-xL, and high expression of Neu and STAT3 in non-small-cell lung carcinoma
Differential express-ion of STAT5 and Bcl-xL, and high expression of Neu and STAT3 in non-small-cell lung carcinoma
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DOI：
10.1016/j.lungcan.2006.07.012
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发表时间：
2006-11-01
期刊：
影响因子：
5.3
通讯作者：
Gutierrez-Castellanos, S.
中科院分区：
文献类型：
--
作者：
Sanchez-Ceja, S. G.;Reyes-Maldonado, E.;Gutierrez-Castellanos, S.
实验证据表明，肺癌的发生、发展和增殖率的增加可能与凋亡相关因素有关。本研究的目的是评估Neu、信号换能器和转录激活因子(STAT)-3、STAT5和Bcl-x(L)在非小细胞肺癌中的表达状况。我们研究了92例非小细胞肺癌标本中这些蛋白的免疫组织化学表达，以确定它们在肺癌发病机制中的作用。Neu在65%的病例中过表达，而STAT3在细胞质中过表达的比例为52.1%，在细胞核（活化）中表达的比例为60.8%。STAT5在细胞质中过表达率为41.3%，在细胞核中过表达率为32.6%。因此，81.5%的Bcl-x(L)在细胞质中过表达。有趣的是，我们在30.4%的病例中发现Bcl-x(L)的核表达。最后，我们发现肺癌的组织学类型与细胞核中STAT5 （P = 0.005）和Bcl-x(L)的表达（P = 0.003）相关。Bcl-x(L)的核表达与TNM分期（远处转移）相关（P=0.02）。这些结果首次提示，STAT5和Bcl-x(L)作为凋亡调节蛋白在肺癌发病过程中的相关作用，以及Neu和活化STAT3的过表达可能与肺癌细胞的增殖率有关。2006爱思唯尔爱尔兰有限公司版权所有。
Experimental evidence suggests that in lung cancer, development, progression and an increased proliferation rate can be linked to apoptosis-related factors. The objective of this study is to evaluate the status of Neu, signal transducer and activator of transcription (STAT)-3, STAT5 and Bcl-x(L) expression in non-small-cell lung cancer. We investigated the immunohistochemical expression of these proteins in 92 non-small-cell lung cancer specimens to establish their rote in lung cancer pathogenesis. Neu was overexpressed in 65% of cases, and although STAT3 was overexpressed in 52.1% in cytoplasm, it was expressed in nucleus (activated) in 60.8%. Meanwhile, STAT5 was found overexpressed in 41.3% in cytoplasm and 32.6% in nucleus. Thus, Bcl-x(L) was overexpressed in cytoplasm in 81.5%. Interestingly, we found nuclear expression of Bcl-x(L) in 30.4% of cases. Finally, we found correlation among histological types of lung cancer and nuclear expression of both STAT5 (P = 0.005) and nuclear Bcl-x(L) (P = 0.003). Besides, nuclear expression of Bcl-x(L) was correlated with TNM stage IV (distant metastasis) (P=0.02). These results suggest for the first time, a relevant role for STAT5 and Bcl-x(L) as apoptosis-regulatory proteins in the pathogenesis of lung cancer, and overexpression of both Neu and activated STAT3, could be related with the proliferation rate in lung carcinoma cells. (C) 2006 Elsevier Ireland Ltd. All rights reserved.