Relationship between the color of carious dentin with varying lesion activity, and bacterial detection
Relationship between the color of carious dentin with varying lesion activity, and bacterial detection
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DOI：
10.1016/j.jdent.2007.11.012
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发表时间：
2008-02-01
影响因子：
4.4
通讯作者：
Ebisu, Shigeyuki
中科院分区：
文献类型：
--
作者：
Iwami, Yukiteru;Hayashi, Norifumi;Ebisu, Shigeyuki
目的：探讨不同病变活动度的龋齿牙本质颜色与病变内细菌检测之间的关系。方法：在26颗拔除的冠状牙本质龋齿和4颗拔除的健康人类磨牙中，用圆车针沿牙髓腔方向，距牙本质表面每隔150μm去除牙本质。去除前后，通过电荷耦合装置（CCD）同时拍摄九色样本和用龋齿检测染料（丙二醇中的1％酸性红）染色的牙本质表面的图像，并用新的圆车针采集牙本质组织样本。根据图像，根据九色样品的颜色变化计算牙本质表面的校正L*、a*和b*值（CIE 1976 L*a*b*颜色系统）。采用聚合酶链式反应检测牙本质组织中的细菌DNA。结果：牙本质去除前，健康磨牙的L*（L*>50）显着大于龋磨牙的L*（L*<SO）（方差分析，Scheffe's F检验，P<0.05）。此外，将龋坏磨牙分为I型（a*>20，活动性龋特征）和II型（a*<20，停滞性龋特征），a*值差异有统计学意义（P<0.05）。对于两种龋类型，L*的接受者操作特征曲线下面积均显着大于a*或b*（单变量Z评分检验，P < 0.05），并且随着牙本质组织L*的增加，细菌检出率降低，而当L*>60时，无法检测到细菌DNA。结论：通过龋齿检测仪染色的牙本质组织的L*和a*值的组合来区分声音和I型和II型龋齿牙本质去除牙本质之前进行染色。在龋损中，染料染色的龋牙本质a*值与龋组织去除前的龋损活动性相关，L*值与龋齿进展程度相关。 (c) 2007 Elsevier Ltd. 保留所有权利。
Objectives: To investigate the relationship between the color of carious dentin with varying lesion activity, and bacterial detection in the lesions.Methods: In 26 extracted human molars with coronal dentin caries and four extracted sound human molars, dentin was removed by a round bur every 150 mu m from the dentin surface, in the direction of the pulp chamber. Before and after removal, images of nine-color samples and the dentin surface stained with a caries detector dye (1% acid red in propylene glycol) were taken simultaneously by a charge-coupled device (CCD), and dentinal tissue samples were taken with a new round bur. From the images, corrected L*, a* and b* values (CIE 1976 L*a*b* color system) of the dentin surfaces were calculated from the color changes of the nine-color samples. Bacterial DNA in the dentinal tissues was detected by polymerase chain reaction.Results: Before removal of dentin, the L* of sound molars (L* > 50) was significantly larger than that of carious molars (L* < SO) (ANOVA, Scheffe's F-test, P < 0.05). In addition, the carious molars were divided into type I (a* > 20, characteristics of active caries) and type II (a* < 20, characteristics of arrested caries), and there was a significant difference in the a* value (P < 0.05). For both carious types, the area under the receiver operating characteristic curve of L* was significantly larger than that of a* or b* (univariate Z score test, P < 0.05), and the rate of bacterial detection decreased as the L* of dentinal tissue increased, and bacterial DNA was not detected when L* was >60.Conclusions: Sound and types I and II carious dentin were discriminated by the combination of L* and a* values of dentinal tissue stained with the caries detector dye before removal of dentin. In carious lesions, the a* values of carious dentin stained with the dye were related to the carious lesion activity before removal of carious tissue, and the L* values were related to the degree of caries progression. (c) 2007 Elsevier Ltd. All rights reserved.