Cloning, heterologous expression, and characterization of recombinant class II cytochromes c from Rhodopseudomonas palustris.
Cloning, heterologous expression, and characterization of recombinant class II cytochromes c from Rhodopseudomonas palustris.
复制标题
沼泽红假单胞菌重组 II 类细胞色素 c 的克隆、异源表达和表征。
DOI：
10.1016/s0304-4165(02)00437-3
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Winkler,JayR
中科院分区：
文献类型：
--
作者：
McGuirl,MicheleA;Lee,JenniferC;Lyubovitsky,JuliaG;Thanyakoop,Chalita;Richards,JohnH;Gray,HarryB;Winkler,JayR
The cytochrome (cyt) c′, cyt c556, and cyt c2genes from Rhodopseudomonas palustris have been cloned; recombinant cyt c′ and cyt c556have been expressed, purified, and characterized. Unlike mitochondrial cyt c, these two proteins are structurally similar to cyt b562, in which the heme is embedded in a four-helix bundle. The hemes in both recombinant proteins form covalent thioether links to two Cys residues. UV/vis spectra of the FeIIand FeIIIstates of the recombinant cyts are identical with those of the corresponding native proteins. Equilibrium unfolding measurements in guanidine hydrochloride solutions confirm that native FeII-cyt c556is more stable than the corresponding state of FeIII-cyt c556(ΔΔGf°=22 kJ/mol).
