The severity of lung injury and metabolic disorders induced by ambient PM2.5 exposure is associated with cumulative dose
The severity of lung injury and metabolic disorders induced by ambient PM2.5 exposure is associated with cumulative dose
复制标题
环境PM2.5暴露引起的肺损伤和代谢紊乱的严重程度与累积剂量相关
DOI：
10.1080/08958378.2018.1508258
复制
发表时间：
2018-01-01
影响因子：
2.1
通讯作者：
Zhao, Jinzhuo
中科院分区：
文献类型：
--
作者：
Jiang, Shuo;Zhou, Ji;Zhao, Jinzhuo
大量的流行病学和实验研究发现，大气细颗粒物（PM2.5）暴露与心肺疾病、肥胖和糖尿病的发生有关。本研究的重点是累积PM2.5暴露对肺部和全身炎症以及胰岛素抵抗的影响。32只6周龄雄性Balb/c小鼠随机分为4组（FA、PM、WEEK和DAY组），采用上海市气象环境动物暴露系统（Shanghai-METAS）连续或间歇暴露于高浓度PM2. 5或过滤空气（FA）4周。检测血清、支气管肺泡灌洗液（BALF）、肺组织和白色脂肪组织（WAT）中IL-6和TNF-α的含量。Western blot检测肺组织NF-B和磷酸化NF-B的表达。在暴露结束时还测定了葡萄糖耐量和胰岛素抵抗。结果发现，PM组小鼠在肺中显示中度炎症细胞浸润，而WEEK和DAY组小鼠在肺中显示轻度炎症细胞浸润。与FA组比较，PM组小鼠肺组织IL-6、TNF-α mRNA表达及WAT均显著升高。PM组IL-6和TNF-α mRNA表达明显高于WEEK组和DAY组.肺组织中磷酸化NF-B蛋白的表达显示PM组NF-B活化，高于WEEK组和DAY组。同时，PM组小鼠表现出比WEEK组和DAY组更严重的糖耐量和胰岛素抵抗。结果表明，减少PM2.5累积暴露可以减轻肺部和脂肪炎症、胰岛素抵抗和糖耐量损害。结果提供了一个线索，即通过室内空气净化等系统中断环境PM2.5暴露可能有益于人们的健康。
Lots of epidemiological and experimental studies have found that ambient fine particulate matter (PM2.5) exposure is associated with the development of cardiopulmonary diseases, obesity and diabetes. This study focused on the effects of cumulative PM2.5 exposure on pulmonary and systemic inflammation and insulin resistance. Thirty-two 6-week-old male Balb/c mice were randomly divided into four groups (FA, PM, WEEK and DAY groups) and were continuously or intermittently exposed to concentrated PM2.5 or filtered air (FA) for four weeks using Shanghai Meteorological and Environmental Animal Exposure System (Shanghai-METAS). The levels of IL-6 and TNF- in serum, bronchoalveolar lavage fluid (BALF), lung tissues and white adipose tissue (WAT) were measured. Meanwhile, the expression of NF-B and phosphor-NF-B in lung tissue was detected by Western blot. Glucose tolerance and insulin resistance were also determined at the end of exposure. The results found that the mice in PM group displayed moderate inflammatory cell infiltration in lung, whereas the mice in WEEK and DAY groups displayed slight inflammatory cell infiltration in lung. Compared with the mice in FA group, the mRNA expressions of IL-6 and TNF- in lung tissue and WAT significantly increased in the mice of PM group. Importantly, IL-6 and TNF- mRNA expressions in PM group were higher than those in WEEK and DAY groups. The protein expression of phospho-NF-B in lung tissue showed that PM group showed the activation of NF-B, which was higher than that in the WEEK and DAY groups. Meanwhile, the mice in PM group showed more severe glucose tolerance and insulin resistance than that in the WEEK and DAY groups. The results suggested that the reduction of PM2.5 cumulative exposure may alleviate pulmonary and adipose inflammation, insulin resistance and glucose tolerance impairment. The results provided a clue that the interruption of ambient PM2.5 exposures by systems such as indoor air purification could be of benefit to people's health.