Comparative analysis of cation/proton antiporter superfamily in plants.
Comparative analysis of cation/proton antiporter superfamily in plants.
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DOI：
10.1016/j.gene.2013.03.104
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发表时间：
2013-06
期刊：
影响因子：
3.5
通讯作者：
C. Ye;Xiaohan Yang;X. Xia;W. Yin
中科院分区：
文献类型：
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作者：
C. Ye;Xiaohan Yang;X. Xia;W. Yin
阳离子/质子逆向转运体超家族与单价阳离子的跨膜运输有关。这个超家族在拟南芥基因组中被注释，并对其中一些成员进行了功能鉴定。本研究对不同植物的阳离子/质子逆向转运蛋白基因进行了系统的分析。我们在藻类、苔藓和被子植物中鉴定出240个阳离子/质子逆向转运蛋白。构建了系统发育树，将这240个成员分为3个家族，即Na+/H+交换体、K+外流逆向转运体和阳离子/H+交换体。我们的分析表明，在所研究的被子植物中，串联和/或节段性复制有助于阳离子/H+交换器的扩展。对非同义/同义替代比的滑动窗口分析表明，阳离子/质子逆向转运体平行基因的进化命运存在一些差异。此外，我们在这240个蛋白质中发现了过度表达的基序，并发现大多数基序是家族特有的，这表明阳离子/质子逆向转运蛋白在三个家族中的进化是不同的。此外，我们还研究了拟南芥中阳离子/质子逆向转运蛋白的共表达基因。结果表明，一些生物过程在共表达的基因中得到了丰富，提示阳离子/质子逆向转运蛋白可能参与了这些生物过程。综上所述，这项研究加深了我们对植物阳离子/质子逆向转运蛋白的认识。
The cation/proton antiporter superfamily is associated with the transport of monovalent cations across membranes. This superfamily was annotated in the Arabidopsis genome and some members were functionally characterized. In the present study, a systematic analysis of the cation/proton antiporter genes in diverse plant species was reported. We identified 240 cation/proton antiporters in alga, moss, and angiosperm. A phylogenetic tree was constructed showing these 240 members are separated into three families, i.e., Na+/H+exchangers, K+efflux antiporters, and cation/H+exchangers. Our analysis revealed that tandem and/or segmental duplications contribute to the expansion of cation/H+exchangers in the examined angiosperm species. Sliding window analysis of the nonsynonymous/synonymous substitution ratios showed some differences in the evolutionary fate of cation/proton antiporter paralogs. Furthermore, we identified over-represented motifs among these 240 proteins and found most motifs are family specific, demonstrating diverse evolution of the cation/proton antiporters among three families. In addition, we investigated the co-expressed genes of the cation/proton antiporters in Arabidopsis thaliana. The results showed some biological processes are enriched in the co-expressed genes, suggesting the cation/proton antiporters may be involved in these biological processes. Taken together, this study furthers our knowledge on cation/proton antiporters in plants.