Maturation of molluscum contagiosum virus (MCV) in vivo: quantitative electron microscopic autoradiography.
Maturation of molluscum contagiosum virus (MCV) in vivo: quantitative electron microscopic autoradiography.
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传染性软疣病毒（MCV）体内成熟：定量电子显微镜放射自显影。
DOI：
10.1111/1523-1747.ep12724146
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
K. Fukuyama
中科院分区：
文献类型：
--
作者：
W. Epstein;K. Fukuyama
为了确定MCV在体内的成熟率，我们将氚化胸苷皮内注射到23个传染性软疣病灶中，并在1小时至8天后间隔进行活检。对组织进行电子显微镜放射自显影处理，并通过在不同时间间隔在发育病毒体上定位银颗粒来确定病毒成熟的模式。结果表明，传染性软疣（MCV）在体内的成熟是异步的。一些病毒粒子在1至2.5小时内迅速成熟，但大多数成熟较慢; 94%在5天内成熟。一项比较研究表明，感染细胞中病毒粒子的成熟在颗粒层比在马氏层发生得更快。在所有层的活表皮细胞中，包括正在分裂的基底细胞中，均观察到新感染细胞中的未包被病毒粒子。在检查的时间间隔内，仅在马尔皮基细胞中观察到“第二阶段脱壳”和“病毒工厂”形成。
To determine the rate of maturation of MCV in vivo, we injected tritiated thymidine intradermally into 23 lesions of molluscum contagiosum and obtained biopsies at intervals from 1 hour to 8 days later. The tissues were processed for electron microscopic autoradiography, and the patterns of viral maturation were determined by the localization of silver grains over developing virions at different time intervals. The findings demonstrate that maturation of molluscum contagiosum (MCV) in vivo is asynchronous. Some virions mature rapidly, within 1 to 2.5 hours, but the majority mature more slowly; 94 percent are mature in 5 days. A comparative study revealed that maturation of virions in infected cells occurred more rapidly in the granular layer than in the malpighian layer. Uncoated virions in newly infected cells were seen in viable epidermal cells in all layers, including dividing basal cells. "Second-stage uncoating" together with "viral factory" formation were observed only in malpighian cells at the time intervals examined.