Vitamin C induced oxidation of eye lens gamma crystallins
Vitamin C induced oxidation of eye lens gamma crystallins
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DOI：
10.1016/s0300-9084(98)80068-0
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发表时间：
1998-04-01
期刊：
影响因子：
3.9
通讯作者：
Slingsby, C
中科院分区：
文献类型：
--
作者：
Atalay, A;Ogus, A;Slingsby, C
晶状体蛋白是眼睛晶状体的长寿命蛋白质，具有维持晶状体透明度的特定结构。众所周知，晶状体蛋白会随着年龄的增长而发生变化，包括氧化。氧化可能会导致白内障的发生。本研究基于聚丙烯酰胺凝胶电泳和电喷雾质谱法研究了金属催化的维生素 C（抗坏血酸）氧化对 γ-晶状体蛋白的影响。当伽马晶体家族的两个成员伽马 B 和伽马 S 受到铜 (II) 和抗坏血酸的攻击时，会发生交联、聚集和变性。这些蛋白质与单独的铜或添加抗坏血酸形成二聚体，这可能是氧化的早期标记。研究发现α-酮戊二酸和丙酮酸在抑制氧化方面非常有效。 (C) 法国生物化学和分子生物学公司/爱思唯尔，巴黎。
Crystallins are long-lived proteins of the eye lens that have specific structures that maintain lens transparency. Lens crystallins are known to undergo changes with age that include oxidation. Oxidation may contribute to cataract development. In this study the effect of metal-catalysed oxidation of vitamin C (ascorbate) on gamma-crystallins was investigated based on polyacrylamide gel electrophoresis and electrospray mass spectrometry. Cross-linking, aggregation and denaturation occurred when two members of the gamma-crystalline family, gamma B and gamma S, were challenged with copper (II) and ascorbate. These proteins form a dimer, with copper alone or with the addition of ascorbate, which may be an early marker of oxidation. It was found that alpha-ketoglutarate and pyruvate were very effective in the inhibition of oxidation. (C) Societe francaise de biochimie et biologie moleculaire/Elsevier, Paris.