Gene expression of MC3T3-E1 cells on fibronectin-immobilized titanium using tresyl chloride activation technique
Gene expression of MC3T3-E1 cells on fibronectin-immobilized titanium using tresyl chloride activation technique
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DOI：
10.4012/dmj.26.647
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发表时间：
2007-09-01
影响因子：
2.5
通讯作者：
Hayakawa, Tohru
中科院分区：
文献类型：
--
作者：
Pugdee, Kamolparn;Shibata, Yasuko;Hayakawa, Tohru
纤维连接蛋白（FN）可以直接固定在钛表面使用三甲苯基氯活化技术。三甲苯基氯活化技术的关键优势在于其简单性。在这项研究中，我们研究了细胞附着和基因表达的MC 3 T3-E1细胞FN固定钛使用基因芯片。与未处理的钛相比，细胞以更高的速率附着在FN固定的钛上。FN改变了基因表达谱，其中62个基因被发现上调，而56个基因被发现下调到第14天水平的两倍以上。FN不仅能增强IBSP和OMD的表达，而且能降低SULF 1 mRNA的表达。两者合计，固定FN上的tresylated钛促进早期基质矿化和骨形成。
Fibronectin (FN) can be immobilized directly on titanium surfaces using tresyl chloride activation technique. The key advantage of tresyl chloride activation technique lies in its simplicity. In this study, we examined the cell attachment and gene expression of MC3T3-E1 cells on FN-immobilized titanium using GeneChip. Cells attached on FN-immobilized titanium at a higher rate than untreated titanium. FN altered the gene expression profile, whereby 62 genes were found to be up-regulated, while 56 genes were found to down-regulate to over twice the level on day 14. FN not only enhanced the expression levels of IBSP and OMD, but also decreased SULF1 mRNA level. Taken together, the immobilization of FN on tresylated titanium promoted early matrix mineralization and bone formation.