Schwann cell-mediated delivery of glial cell line-derived neurotrophic factor restores erectile function after cavernous nerve injury
Schwann cell-mediated delivery of glial cell line-derived neurotrophic factor restores erectile function after cavernous nerve injury
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DOI：
10.1111/iju.12078
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发表时间：
2013-03-01
影响因子：
2.6
通讯作者：
Matiasek, Kaspar
中科院分区：
文献类型：
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作者：
May, Florian;Buchner, Alexander;Matiasek, Kaspar
目的探讨神经营养因子转导的神经细胞硅管对海绵状神经损伤后功能恢复的时间过程。方法双侧切除海绵状神经（5mm），即刻双侧手术修复。每组共重建20条研究神经，分别置入空硅管和硅管，硅管中分别播种过表达神经营养因子的胶质细胞系衍生的或表达绿色荧光蛋白的雪旺细胞。对照组采用假手术或双侧神经横断，不进行神经重建。术后2周、4周、6周、8周、10周分别通过开腹、神经电刺激和海绵内压记录评估勃起功能。这些动物只进行了一次重新探索，之后就被杀死了。观察神经移植物再生神经纤维的成熟状态和神经束组成。结果大鼠勃起功能恢复时间至少为4周。胶质细胞系衍生的神经营养因子转导的雪旺细胞移植物比绿色荧光蛋白转导的对照和未播种的导管更能恢复勃起功能。神经胶质细胞系衍生的神经营养因子转导的移植物在4、6、8和10周时促进了完整的勃起反应（4/4），总体上显著优于阴性对照组(P
Objectives To evaluate the time-course of functional recovery after cavernous nerve injury using glial cell line-derived neurotrophic factor-transduced Schwann cell-seeded silicon tubes. Methods Sections of the cavernous nerves were excised bilaterally (5mm), followed by immediate bilateral surgical repair. A total of 20 study nerves per group were reconstructed by interposition of empty silicon tubes and silicon tubes seeded with either glial cell line-derived neurotrophic factor-overexpressing or green fluorescent protein-expressing Schwann cells. Control groups were either sham-operated or received bilateral nerve transection without nerve reconstruction. Erectile function was evaluated by relaparotomy, electrical nerve stimulation and intracavernous pressure recording after 2, 4, 6, 8 and 10 weeks. The animals underwent re-exploration only once, and were killed afterwards. The nerve grafts were investigated for the maturation state of regenerating nerve fibers and the fascular composition. Results Recovery of erectile function took at least 4 weeks in the current model. Glial cell line-derived neurotrophic factor-transduced Schwann cell grafts restored erectile function better than green fluorescent protein-transduced controls and unseeded conduits. Glial cell line-derived neurotrophic factor-transduced grafts promoted an intact erectile response (4/4) at 4, 6, 8 and 10 weeks that was overall significantly superior to negative controls (P