Analysis of the virus population present in equine faeces indicates the presence of hundreds of uncharacterized virus genomes
Analysis of the virus population present in equine faeces indicates the presence of hundreds of uncharacterized virus genomes
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DOI：
10.1007/s11262-004-5624-3
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发表时间：
2005-03-01
期刊：
影响因子：
1.6
通讯作者：
Heaphy, S
中科院分区：
文献类型：
--
作者：
Cann, AJ;Fandrich, SE;Heaphy, S
从马粪便中分离病毒DNA，并以不依赖序列的方式克隆.对268个克隆进行测序，获得178140个核苷酸的序列。统计分析表明，该文库包含来自多达233个不同病毒基因组的17560个不同克隆。TBLASTX分析表明，32%的克隆与GenBank中的序列有显著的同源性。其中63%是病毒，20%是细菌，7%是古细菌，6%是真核生物，5%与移动的遗传因素有关。52%的病毒同一性与Siphoviridae; 26%未分类的病毒; 17%肌病毒科; 4%短尾病毒科;和一个克隆（2%）是脊椎动物正痘病毒。编码预测的病毒结构蛋白、蛋白酶、糖苷酶和核酸结合蛋白的基因是常见的。
Virus DNA was isolated from horse faeces and cloned in a sequence- independent fashion. 268 clones were sequenced and 178140 nucleotides of sequence obtained. Statistical analysis suggests the library contains 17560 distinct clones derived from up to 233 different virus genomes. TBLASTX analysis showed that 32% of the clones had significant identity to GenBank entries. Of these 63% were viral; 20% bacterial; 7% archaeal; 6% eukarya; and 5% were related to mobile genetic elements. Fifty-two percent of the virus identities were with Siphoviridae; 26% unclassified phages; 17% Myoviridae; 4% Podoviridae; and one clone (2%) was a vertebrate Orthopoxvirus. Genes coding for predicted virus structural proteins, proteases, glycosidases and nucleic acid-binding proteins were common.