Arabidopsis RopGEF4 and RopGEF10 are important for FERONIA-mediated developmental but not environmental regulation of root hair growth
Arabidopsis RopGEF4 and RopGEF10 are important for FERONIA-mediated developmental but not environmental regulation of root hair growth
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拟南芥 RopGEF4 和 RopGEF10 对 FERONIA 介导的根毛生长发育很重要，但对环境调节不重要
DOI：
10.1111/nph.12432
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发表时间：
2013-12-01
期刊：
影响因子：
9.4
通讯作者：
Zhang, Yan
中科院分区：
文献类型：
--
作者：
Huang, Guo-Qiang;Li, En;Zhang, Yan
我们研究了拟南芥根毛发育中的一条遗传途径，涉及到受体样激酶FERONIA（FER），两个ROP的鸟嘌呤核苷酸交换因子功能丧失和获得分析证明了RopGEF 4和RopGEF 10的不同作用，使得RopGEF 4仅对根毛伸长重要，而RopGEF 10主要参与根毛的发生。通过截断和结构域交换的结构域切割实验表明，它们的功能差异主要是由非催化结构域贡献的，使用荧光比率成像，我们表明，功能的损失RopGEF 4和RopGEF 10相加减少活性氧（ROS）的产生。双分子荧光互补实验表明，RopGEF 4和RopGEF 10与ROP具有相同的互作特异性，表明它们具有共同的下游组分，并进一步表明外源生长素和磷限制等环境因素对根毛发育的促进作用依赖于FER。然而，尽管RopGEF4和RopGEF10的功能丧失在很大程度上消除了FER诱导的ROS产生，但它并没有损害对FER介导的环境线索对根毛发育的反应。我们的研究结果表明，RopGEF4和RopGEF10是FER介导的，发育（但不是环境）调节，根毛生长的遗传成分。
We investigated a genetic pathway in root hair development in Arabidopsis thaliana, involving the receptor-like kinase FERONIA (FER), two guanine nucleotide exchange factors for ROPs (RopGEF4 and RopGEF10), and the small GTPase Rho of plants (ROPs).Loss- and gain-of-function analyses demonstrated distinct roles of RopGEF4 and RopGEF10 such that RopGEF4 is only important for root hair elongation, while RopGEF10 mainly contributes to root hair initiation. Domain dissection by truncation and domain-swapping experiments indicated that their functional distinctions were mainly contributed by the noncatalytic domains.Using fluorescent ratio imaging, we showed that functional loss of RopGEF4 and RopGEF10 additively reduced reactive oxygen species (ROS) production. Bimolecular fluorescence complementation experiments demonstrated that RopGEF4 and RopGEF10 had the same interaction specificity as ROPs, suggesting common downstream components.We further showed that the promoting effects of environmental cues such as exogenous auxin and phosphate limitation on root hair development depended on FER. However, although functional loss of RopGEF4 and RopGEF10 largely abolished FER-induced ROS production, it did not compromise the responses to FER-mediated environmental cues on root hair development. Our results demonstrated that RopGEF4 and RopGEF10 are genetic components in FER-mediated, developmentally (but not environmentally) regulated, root hair growth.