Conditional rhythmicity of ventral spinal interneurons defined by expression of the Hb9 homeodomain protein
Conditional rhythmicity of ventral spinal interneurons defined by expression of the Hb9 homeodomain protein
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DOI：
10.1523/jneurosci.0274-05.2005
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发表时间：
2005-06-15
影响因子：
5.3
通讯作者：
Brownstone, RM
中科院分区：
文献类型：
--
作者：
Wilson, JM;Hartley, R;Brownstone, RM
作为节律性运动网络基础的哺乳动物脊髓中间神经元的特性仍然知之甚少。使用出生后转基因小鼠（其中绿色荧光蛋白的表达由同源结构域转录因子 Hb9 的启动子驱动）以及 Hb9 - lacZ 敲入小鼠，我们描述了位于从颈椎到中腰脊髓水平的腹侧连合附近的新型谷氨酸能中间神经元群体。 Hb9(+) 中间神经元表现出强烈的抑制后反弹，并在响应诱导运动活动的化学刺激时表现出明显的膜电位振荡。这些数据提供了一小群腹侧脊髓中间神经元的分子和生理学轮廓，这些中间神经元表现出均匀的电生理特征，其特性表明它们是候选的运动节律生成中间神经元。
The properties of mammalian spinal interneurons that underlie rhythmic locomotor networks remain poorly described. Using postnatal transgenic mice in which expression of green fluorescent protein is driven by the promoter for the homeodomain transcription factor Hb9, as well as Hb9 - lacZ knock-in mice, we describe a novel population of glutamatergic interneurons located adjacent to the ventral commissure from cervical to midlumbar spinal cord levels. Hb9(+) interneurons exhibit strong postinhibitory rebound and demonstrate pronounced membrane potential oscillations in response to chemical stimuli that induce locomotor activity. These data provide a molecular and physiological delineation of a small population of ventral spinal interneurons that exhibit homogeneous electrophysiological features, the properties of which suggest that they are candidate locomotor rhythm-generating interneurons.