C-13-NMR REVEALS GLYCEROL AS AN UNEXPECTED MAJOR METABOLITE OF THE PROTOZOAN PARASITE TRICHOMONAS-VAGINALIS
C-13-NMR REVEALS GLYCEROL AS AN UNEXPECTED MAJOR METABOLITE OF THE PROTOZOAN PARASITE TRICHOMONAS-VAGINALIS
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DOI：
10.1016/0014-5793(85)80026-0
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发表时间：
1985-01-01
期刊：
影响因子：
3.5
通讯作者：
WILLIAMS, J
中科院分区：
文献类型：
--
作者：
CHAPMAN, A;LINSTEAD, DJ;WILLIAMS, J
用~（13）C-NMR研究了阴道毛滴虫（Trichomonasvaginalis）完整细胞在厌氧培养过程中[1-~（13）C]葡萄糖代谢产物的形成动力学。除了预期的代谢产物乳酸盐和乙酸盐外，该技术还显示甘油是一种额外的主要产物，其含量与乙酸盐等摩尔。甘油的形成很容易解释为需要保持氧化还原平衡。这种原生动物现在加入了一小群生物体，已知这些生物体由于正常的代谢活动而产生甘油。
13C-NMR has been used to study the kinetics of the formation of metabolites from [l-13C]glucose in intact cells ofTrichomonas vaginalisduring anaerobic incubation. As well as the expected metabolites lactate and acetate, this technique revealed glycerol as an additional major product, present in amounts equimolar with acetate. The formation of glycerol is readily explained in terms of the need to maintain redox balance. This protozoan now joins the small group of organisms which are known to produce glycerol as a result of normal metabolic activities.