High-throughput screening for novel anti-infectives using a C. elegans pathogenesis model.
High-throughput screening for novel anti-infectives using a C. elegans pathogenesis model.
复制标题
DOI：
10.1002/9780470559277.ch130160
复制
发表时间：
2014-03-14
影响因子：
--
通讯作者：
Kirienko, Natalia V
中科院分区：
文献类型：
--
作者：
Conery, Annie L;Larkins-Ford, Jonah;Ausubel, Frederick M;Kirienko, Natalia V
在最近的历史中，线虫秀丽隐杆线虫为发现新型抗微生物药物提供了一个引人注目的平台。在这个协议中，我们提出了一个自动化的，高通量的C。elegans致病性测定，其可用于筛选防止线虫由于铜绿假单胞菌而死亡的抗感染化合物。从这些筛选中鉴定出的新抗生素将是治疗人类感染的有希望的候选物，并且还可以用作探针化合物以鉴定微生物发病机理或宿主免疫中的新靶标。
In recent history, the nematode Caenorhabditis elegans has provided a compelling platform for the discovery of novel antimicrobial drugs. In this protocol, we present an automated, high-throughput C. elegans pathogenesis assay, which can be used to screen for anti-infective compounds that prevent nematodes from dying due to Pseudomonas aeruginosa. New antibiotics identified from such screens would be promising candidates for treatment of human infections, and also can be used as probe compounds to identify novel targets in microbial pathogenesis or host immunity.