NADPH oxidases and the evolution of plant salinity tolerance
NADPH oxidases and the evolution of plant salinity tolerance
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NADPH氧化酶与植物耐盐性的进化
DOI：
10.1111/pce.13907
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发表时间：
2020-10-26
影响因子：
7.3
通讯作者：
Shabala, Sergey
中科院分区：
文献类型：
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作者：
Liu, Minmin;Yu, Huiyang;Shabala, Sergey
土壤盐碱化是对全球粮食安全和自然生态系统生物多样性的主要威胁。为了适应盐胁迫，植物依赖于ROS介导的信号传导网络，该网络在广泛的生理和遗传过程的上游操作。ROS信号传导中的一个关键参与者是NADPH氧化酶，一种由RBOH基因编码的质膜结合酶。在这项研究中，我们已经进行了全面的生物信息学分析，超过50盐生植物和glycophytic物种连接的ROS信号转导动力学的差异与NADPH氧化酶的丰度和/或结构之间的对比物种。除了红藻门、绿藻门和链藻门的一些藻类物种外，所有测试的植物谱系中都预测了RBOH蛋白。在glycophytic组中，RBOH拷贝数与盐胁迫耐受性呈负相关，这表明RBOH异构体数量的减少可能与植物耐盐性的进化有关。虽然盐生植物在进化过程中没有发展出独特的蛋白质家族，但它们在NADPH氧化酶的N-末端进化出了额外的磷酸化靶位点，潜在地调节酶活性并允许对其功能进行更多的控制，从而导致更有效的ROS信号传导和对盐条件的适应。
Soil salinization is a major threat to global food security and the biodiversity of natural ecosystems. To adapt to salt stress, plants rely on ROS-mediated signalling networks that operate upstream of a broad array of physiological and genetic processes. A key player in ROS signalling is NADPH oxidase, a plasma-membrane-bound enzyme encoded by RBOH genes. In this study, we have conducted a comprehensive bioinformatic analysis of over 50 halophytic and glycophytic species to link the difference in the kinetics of ROS signalling between contrasting species with the abundance and/or structure of NADPH oxidases. The RBOH proteins were predicted in all the tested plant lineages except some algae species from the Rhodophyta, Chlorophyta and Streptophyta. Within the glycophytic group, the number of RBOH copies correlated negatively with salinity stress tolerance, suggesting that a reduction in the number of RBOH isoforms may be potentially related to the evolution of plant salinity tolerance. While halophytes did not develop unique protein families during evolution, they evolved additional phosphorylation target sites at the N-termini of NADPH oxidases, potentially modulating enzyme activity and allowing more control over their function, resulting in more efficient ROS signalling and adaptation to saline conditions.