In Vivo Interference with AtTCP20 Function Induces Severe Plant Growth Alterations and Deregulates the Expression of Many Genes Important for Development
In Vivo Interference with AtTCP20 Function Induces Severe Plant Growth Alterations and Deregulates the Expression of Many Genes Important for Development
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DOI：
10.1104/pp.108.126136
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发表时间：
2009-03-01
期刊：
影响因子：
7.4
通讯作者：
Tremousaygue, Dominique
中科院分区：
文献类型：
--
作者：
Herve, Christine;Dabos, Patrick;Tremousaygue, Dominique
AtTCP20 是属于拟南芥 (Arabidopsis thaliana) TCP-P 亚家族的转录因子，其特征在于其能够结合位点 II 基序 (TGGGCY)。我们的目的是了解 AtTCP20 在植物发育中的作用。 Prom(TCP20):CDS20::GUS::GFP 翻译融合体的表达模式表明 AtTCP20 在多个植物器官和发育阶段中具有功能。 AtTCP20 的作用在植物中受到诱导与转录激活域 (VP16) 或阻遏域 (EAR) 融合的 AtTCP20 蛋白表达的挑战。两种修饰蛋白的表达都会导致严重的发育表型。深入分析表明AtTCP20可能参与细胞扩增、细胞分裂和细胞分化的调控。根和下胚轴的基因表达谱显示，在 AtTCP20::EAR 阻遏基因诱导后，两个器官中有 252 个基因下调。位点 II 基序 (TGGGCY) 在其启动子中代表性不足。相反，与水稻 (Oryza sativa) 中 TCP 蛋白结合位点相关的 GG(A/T) CCC 序列被过度呈现，并且 TCP20 融合蛋白显示在体外与这些序列结合。基因本体论表明，许多靶基因参与细胞壁生物合成和扩增过程中的修饰，并且还编码许多控制植物发育的转录因子。我们的结果与之前的提议一致，即AtTCP20参与细胞分裂和生长协调。此外，他们进一步表明AtTCP20还有助于细胞扩张控制，并表明该蛋白在植物形态发生中的不同参与取决于器官和发育阶段。
AtTCP20 is a transcription factor belonging to the Arabidopsis (Arabidopsis thaliana) TCP-P subfamily, characterized by its capacity to bind to site II motifs (TGGGCY). Our aim was to understand the role of AtTCP20 in plant development. The expression pattern of a translational fusion of Prom(TCP20):CDS20::GUS::GFP suggested a function for AtTCP20 in several plant organs and stages of development. The role of AtTCP20 was challenged in planta by inducing expression of AtTCP20 proteins fused with either a transcriptional activator domain (VP16) or a repressor domain (EAR). Expression of both modified proteins led to severe developmental phenotypes. In-depth analysis suggested that AtTCP20 may participate in the regulation of cell expansion, cell division, and cell differentiation. Gene expression profiling in roots and hypocotyls revealed that 252 genes were down-regulated in both organs after induction of the AtTCP20::EAR repressor gene. Site II motifs (TGGGCY) were underrepresented in their promoters. Conversely, GG(A/T) CCC sequences related to binding sites identified for TCP proteins in rice (Oryza sativa) were overrepresented, and a TCP20 fusion protein was shown to bind to these sequences in vitro. Gene ontology indicated that many targeted genes were involved in cell wall biogenesis and modification during expansion and also encoded numerous transcription factors controlling plant development. Our results are consistent with the previous proposal that AtTCP20 is involved in cell division and growth coordination. Moreover, they further suggest that AtTCP20 also contributes to cell expansion control and indicate a different involvement of this protein in plant morphogenesis depending on the organ and the developmental stage.