The amino acid motif L/IIxxFE defines a novel actin-binding sequence in PDZ-RhoGEF.
The amino acid motif L/IIxxFE defines a novel actin-binding sequence in PDZ-RhoGEF.
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DOI：
10.1021/bi9010013
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发表时间：
2009-08-25
期刊：
影响因子：
2.9
通讯作者：
Wedegaertner PB
中科院分区：
文献类型：
--
作者：
Banerjee J;Fischer CC;Wedegaertner PB
PDZ-RhoGEF is a member of the regulator of G protein signaling (RGS) domain-containing RhoGEFs (RGS-RhoGEFs) that link activated heterotrimeric G protein α subunits of the G12 family to activation of the small GTPase RhoA. Unique among the RGS-RhoGEFs, PDZ-RhoGEF contains a short sequence that localizes the protein to the actin cytoskeleton. In this report, we demonstrate that the actin-binding domain, located between amino acids 561–585, directly binds to F-actin in vitro. Extensive mutagenesis identifies isoleucine 568, isoleucine 569, phenylalanine 572, and glutamic acid 573 as necessary for binding to actin and for co-localization with the actin cytoskeleton in cells. These results define a novel actin-binding sequence in PDZ-RhoGEF with a critical amino acid motif of IIxxFE. Moreover, sequence analysis identifies a similar actin-binding motif in the N-terminus of the RhoGEF frabin, and, as with PDZ-RhoGEF, mutagenesis and actin interaction experiments demonstrate a motif of LIxxFE, consisting of the key amino acids leucine 23, isoleucine 24, phenylalanine 27, and glutamic acid 28. Taken together, results with PDZ-RhoGEF and frabin identify a novel actin binding sequence. Lastly, inducible dimerization of the actin-binding region of PDZ-RhoGEF revealed a dimerization-dependent actin bundling activity in vitro. PDZ-RhoGEF exists in cells as a dimer, raising the possibility that PDZ-RhoGEF could influence actin structure independent of its ability to activate RhoA.
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影响因子：
3.3
作者：
Banerjee, J;Wedegaertner, PB
通讯作者：
Wedegaertner, PB
影响因子：
64.5
作者：
RIDLEY, AJ;PATERSON, HF;HALL, A
通讯作者：
HALL, A
影响因子：
4
作者：
Buchsbaum, Rachel J.
通讯作者：
Buchsbaum, Rachel J.
DOI：
10.1073/pnas.0701656104
发表时间：
2007-04-10
影响因子：
11.1
作者：
Okamoto, Ken-Ichi;Narayanan, Radhakrishnan;Hayashi, Yasunori
通讯作者：
Hayashi, Yasunori
影响因子：
4.8
作者：
Reuther, GW;Lambert, QT;Der, CJ
通讯作者：
Der, CJ