Postprandial nutrient-sensing and metabolic responses after partial dietary fishmeal replacement by soyabean meal in turbot (Scophthalmus maximus L.)
Postprandial nutrient-sensing and metabolic responses after partial dietary fishmeal replacement by soyabean meal in turbot (Scophthalmus maximus L.)
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DOI：
10.1017/s0007114515004535
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发表时间：
2015-11
影响因子：
3.6
通讯作者：
Dandan Xu;G. He;K. Mai;Huihui Zhou;Wei Xu;Fei Song
中科院分区：
文献类型：
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作者：
Dandan Xu;G. He;K. Mai;Huihui Zhou;Wei Xu;Fei Song
摘要本研究以大菱鲆（Scophthalmus maximus L.）以鱼粉（FM）或豆粕替代45%的FM（34.6%干饲料），添加或不添加必需氨基酸（EAA），观察其营养感受和代谢反应。经过1个月的饲养试验，添加豆粕（SMI）的饲料显著降低了鱼的生长、饲料效率和营养物质存留率。与FM饮食相比，SMI导致游离氨基酸的餐后流入减少，雷帕霉素信号传导的低活化靶点和再喂养后的高活化氨基酸反应途径，与蛋白质合成减少，餐后糖酵解和脂肪生成受损相关的状态。这些差异的影响并没有改善匹配的EAA配置文件的豆粕FM饮食，通过膳食氨基酸补充剂。因此，这项研究表明，FM饮食和SMI饮食导致不同的营养感应反应，这反过来又调节代谢，并确定饮食的利用效率。我们的研究结果提供了一个新的分子解释的营养传感的作用，在水产饲料中，FM是由豆粕替代性能较差。
Abstract In this study, we chose a carnivorous fish, turbot (Scophthalmus maximus L.), to examine its nutrient-sensing and metabolic responses after ingestion of diets with fishmeal (FM), or 45 % of FM replaced by soyabean meal (34·6 % dry diet) balanced with or without essential amino acids (EAA) to match the amino acid profile of FM diet for 30 d. After a 1-month feeding trial, fish growth, feed efficiency and nutrient retention were markedly reduced by soyabean meal-incorporated (SMI) diets. Compared with the FM diet, SMI led to a reduction of postprandial influx of free amino acids, hypoactivated target of rapamycin signalling and a hyperactivated amino acid response pathway after refeeding, a status associated with reduced protein synthesis, impaired postprandial glycolysis and lipogenesis. These differential effects were not ameliorated by matching an EAA profile of soyabean meal to that of the FM diet through dietary amino acid supplementation. Therefore, this study demonstrated that the FM diet and SMI diets led to distinct nutrient-sensing responses, which in turn modulated metabolism and determined the utilisation efficiency of diets. Our results provide a new molecular explanation for the role of nutrient sensing in the inferior performance of aquafeeds in which FM is replaced by soyabean meal.