Localization of Tricellular Tight Junction Molecule LSR at Midbody and Centrosome During Cytokinesis in Human Epithelial Cells
Localization of Tricellular Tight Junction Molecule LSR at Midbody and Centrosome During Cytokinesis in Human Epithelial Cells
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DOI：
10.1369/0022155419886263
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发表时间：
2019-10-29
影响因子：
3.2
通讯作者：
Kojima, Takashi
中科院分区：
文献类型：
--
作者：
Konno, Takumi;Kohno, Takayuki;Kojima, Takashi
在脊椎动物上皮组织胞质分裂期间维持上皮完整性和屏障功能。三细胞紧密连接分子脂解刺激的脂蛋白受体（LSR）在胞质分裂过程中的定位和作用的变化还不清楚，虽然新的三细胞紧密连接在胞质分裂过程中的中体侧翼形成。本研究以人子宫内膜癌细胞株Sawano为材料，比较了三细胞紧密连接分子tricellulin、双细胞紧密连接分子occludin、claudin-7、zonula occludens-1和cingulin在胞质分裂过程中的定位变化及其在中间体和中心体中的作用。以及上皮极化相关分子p53蛋白2、PAR 3和yes相关蛋白。在细胞质分裂过程中诱导的治疗与紫杉醇，上皮屏障得以维持和三细胞紧密连接分子LSR和tricellulin集中在侧翼的乙酰化微管蛋白阳性的中间体和γ-微管蛋白阳性中心体与动力蛋白适配器钩2，而其他分子也定位在那里。所有的分子都通过使用小干扰RNA敲低而消失。此外，通过敲低Hook 2，上皮屏障得以维持，并且大多数分子从中心体消失。这些发现表明LSR可能不仅在屏障功能中而且在胞质分裂中起关键作用。
Epithelial integrity and barrier function are maintained during cytokinesis in vertebrate epithelial tissues. The changes in localization and the roles of tricellular tight junction molecule lipolysis-stimulated lipoprotein receptor (LSR) during cytokinesis are not well known, although new tricellular tight junctions form at the flank of the midbody during cytokinesis. In this study, we investigated the changes in localization and the role of LSR at the midbody and centrosome during cytokinesis using human endometrial carcinoma cell line Sawano, comparing the tricellular tight junction molecule tricellulin; bicellular tight junction molecules occludin, claudin-7, zonula occludens-1, and cingulin; and the epithelial polarized related molecules apoptosis-stimulating of p53 protein 2, PAR3, and yes-associated protein. During cytokinesis induced by treatment with taxol, the epithelial barrier was maintained and the tricellular tight junction molecules LSR and tricellulin were concentrated at the flank of the acetylated tubulin-positive midbody and in gamma-tubulin-positive centrosomes with the dynein adaptor Hook2, whereas the other molecules were localized there as well. All the molecules disappeared by knockdown using small interfering RNAs. Furthermore, by the knockdown of Hook2, the epithelial barrier was maintained and most of the molecules disappeared from the centrosome. These findings suggest that LSR may play crucial roles not only in barrier function but also in cytokinesis.