The total flavones from Semen cuscutae reverse the reduction of testosterone level and the expression of androgen receptor gene in kidney-yang deficient mice
The total flavones from Semen cuscutae reverse the reduction of testosterone level and the expression of androgen receptor gene in kidney-yang deficient mice
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DOI：
10.1016/j.jep.2008.06.027
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发表时间：
2008-09-02
影响因子：
5.4
通讯作者：
Guo, Juan
中科院分区：
文献类型：
--
作者：
Yang, Jianxiong;Wang, Yali;Guo, Juan
目的：探讨藜芦子总黄酮(TFSC)对肾阳虚雄性小鼠激素水平及肾、睾丸雄激素受体(AR)基因和蛋白水平的影响。方法：ICR雄性小鼠随机分为6组，除正常组外，均给予氢化可的松造成肾阳虚模型。除正常组和对照组外，其余各组均给予TFSC、甲睾酮和金匮肾气丸治疗。结果：对照组大鼠血清睾酮水平、AR mRNA水平和蛋白水平均低于对照组，TFSC可逆转氢化可的松引起的睾酮水平、AR mRNA水平和蛋白水平的降低。结论：TFSC可通过恢复肾、睾丸组织睾酮水平和AR mRNA及蛋白表达水平，从而逆转肾阳虚证大鼠的肾阳虚症状。(C)2008爱思唯尔爱尔兰有限公司。保留所有权利。
Objective: To discover the effect of the total flavones from Semen cuscutae (TFSC) on the kidney-yang deficiency male mouse, especially on hormone levels and the androgen receptor (AR) mRNA and protein level in the kidney and testicle.Methods: ICR male mouse were separated into six groups, and, except for the normal group, hydrocortisone was injected intraperitoneally to make the kidney-yang deficiency. The groups were then treated with TFSC, methyltestosterone and Jinkui Shenqi Wan, except for the normal group and control group. 14 days after administering, testosterone levels in the total serum were analysed by radioimmunoassay, AR mRNA levels measured by real time RT-PCR and protein levels by immunohistochemistry.Results: The control group had the lowest testosterone level, AR mRNA level and protein level, and the TFSC can reverse the reduction of testosterone level, AR mRNA level and protein level induced by the hydrocortisone.Conclusions: This is the first study to demonstrate that TFSC treatment may reverse kidney-yang deficiency symptoms by restoring the levels of testosterone and AR mRNA and protein expression in the kidney and testicle. (C) 2008 Elsevier Ireland Ltd. All rights reserved.