Synergy of CO2 Response and Aggregation-Induced Emission in a Block Copolymer: A Facile Way To "See" Cancer Cells
Synergy of CO2 Response and Aggregation-Induced Emission in a Block Copolymer: A Facile Way To "See" Cancer Cells
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嵌段共聚物中 CO2 响应和聚集诱导发射的协同作用：“看到”癌细胞的简便方法
DOI：
10.1021/acsami.9b11945
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发表时间：
2019
影响因子：
9.5
通讯作者：
Wang Yao
中科院分区：
文献类型：
--
作者：
Li Yao;Wu Xin;Yang Bin;Zhang Xiaokai;Li Hao;Umar Ahmad;de Rooij Nicolaas Frans;Zhou Guofu;Wang Yao
二氧化碳（CO2）是三羧酸循环产生的重要气体分子代谢物，是识别细胞和组织中癌症的直接信号。本文报道了一种新型“可呼吸”嵌段聚合物超分子组装探针的设计和合成，该探针由亲水性嵌段、含脒的CO2响应性嵌段和聚集诱导发射（AIE）发光块组成，用于检测癌细胞代谢的CO2。成功合成并表征了三嵌段共聚物聚-(4-十一烷氧基四苯乙烯甲基丙烯酸酯)-b-聚-((N-脒基)-(2,3-二氢-1H-1,4-甲基-1,2,3-三唑)-(乙烯基苯))-b-聚环氧乙烷(PTPE-b-PAD-b-PEO)。这种三嵌段共聚物可以自组装成“透气”的水溶液囊泡。在 CO2 存在的情况下，囊泡中含有脒的 CO2 响应块（PAD 块）会“吸入”一定量的 CO2，从而导致囊泡的体积膨胀。囊泡的扩张诱导AIE发光块（PTPE块）的聚集，从而导致荧光强度增强。超分子囊泡“呼出”CO2，当溶液中通入N2时，囊泡的体积和AIE现象减小。基于这种荧光强度的可逆性变化，HeLa宫颈癌细胞、CNE1鼻咽癌细胞、5-8F鼻咽癌细胞、16HBE人支气管上皮细胞、GES-1人胃粘膜上皮细胞均被成功检测和鉴定。
Carbon dioxide (CO2), an important gas molecule metabolite produced by the tricarboxylic acid cycle, is a direct signal for identifying cancers in cells and tissues. Herein, design and synthesis of a novel “breathable” block polymer supramolecular assembly probe consisting of a hydrophilic block, an amidine-containing CO2-responsive block, and an aggregation-induced emission (AIE) luminescence block to detect CO2metabolized by cancer cells is reported. The triblock copolymer poly-(4-undecoxy tetraphenyl ethylene methacrylate)-b-poly-((N-amidino)-(2,3-dihydro-1H-1, 4-methyl-1, 2,3-triazole)-(ethenylbenzene))-b-poly(ethylene oxide) (PTPE-b-PAD-b-PEO) was successfully synthesized and characterized. This triblock copolymer could be self-assembled into “breathable” aqueous solution vesicles. In the presence of CO2, the amidine-containing CO2-responsive block (PAD block) of the vesicle “inhales” an amount of CO2, which causes the volume of the vesicle to expand. The expansion of the vesicle induces the aggregation of the AIE luminescence block (PTPE block), which resulted in the fluorescence intensity enhancement. The supramolecular vesicles “exhale” CO2, and the volume and AIE phenomenon of the vesicles decrease when N2is passed into the solution. On the basis of this reversible change of fluorescence intensity, HeLa cervical cancer cells, CNE1 nasopharynx cancer cells, 5-8F nasopharynx cancer cells, 16HBE human bronchial epithelial cells, and GES-1 human gastric mucosa epithelial cells have all been successfully detected and identified.