Investigation of the Anti-HIV Properties of Oxihumate
Investigation of the Anti-HIV Properties of Oxihumate
复制标题
氧化腐殖酸抗 HIV 特性的研究
DOI：
10.1159/000064919
复制
发表时间：
2002
期刊：
影响因子：
3.3
通讯作者：
Johann W. Schneider
中科院分区：
文献类型：
--
作者：
C. E. J. V. Rensburg;J. Dekker;Roland Weis;Tracey;E. J. V. Rensburg;Johann W. Schneider
开发了一种独特的工艺，通过控制湿氧化，然后进行碱处理，将烟煤转化为水溶性腐植酸盐，称为oxihumate。氧化腐植酸盐抑制MT-2细胞的HIV-1感染，IC 50为12.5 µg/ml。用oxihumate治疗游离和细胞附着的HIV不可逆地降低了感染性，而靶细胞对病毒的易感性在感染前并没有受到治疗的影响。HIV颗粒的感染性通过干扰CD 4结合和V3环介导的病毒进入步骤而被抑制。在体外12周内，没有对oxihumate产生病毒耐药性。因此，氧化腐植酸盐有望用于治疗艾滋病毒感染者。
A unique process has been developed to convert bituminous coal by controlled wet oxidation followed by base treatment to a water-soluble humate called oxihumate. Oxihumate inhibited HIV-1 infection of MT-2 cells with an IC50 of 12.5 µg/ml. Treatment of free and cell-attached HIV with oxihumate irreversibly reduced infectivity, while the susceptibility of target cells to the virus was not impaired by treatment prior to infection. The infectivity of the HIV particles was inhibited by interference with CD4 binding and the V3 loop-mediated step of virus entry. No viral resistance to oxihumate developed over a 12-week period in vitro. Oxihumate therefore holds promise for the treatment of HIV-infected patients.