OsNRAMP2 facilitates Cd efflux from vacuoles and contributes to the difference in grain Cd accumulation between japonica and indica rice
OsNRAMP2 facilitates Cd efflux from vacuoles and contributes to the difference in grain Cd accumulation between japonica and indica rice
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OsNRAMP2促进Cd从液泡中流出并导致粳稻和籼稻籽粒Cd积累的差异
DOI：
10.1016/j.cj.2022.09.013
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发表时间：
2022-10
期刊：
影响因子：
6.6
通讯作者：
Bin Liu
中科院分区：
文献类型：
--
作者：
Wu Yang;Luo Chen;Yamei Ma;Rui Hu;Jian Wang;Wenhui Li;Jingfang Dong;Tifeng Yang;Lian Zhou;Jiansong Chen;Dilin Liu;Ning Yu;Zhixia Liu;Lingyan Zhou;Shaohong Zhang;Junliang Zhao;Bin Liu
镉在水稻籽粒中的积累是一个健康问题。在本研究中，敲除NRAMP家族成员OsNRAMP 2使籽粒Cd浓度降低了38%以上，过表达OsNRAMP 2使籽粒Cd浓度增加了50%以上。生理实验表明，OsNRAMP2通过正调控Cd从液泡的流出，促进Cd从根向地上部的转运。在灌浆期，OsNRAMP 2在除谷壳外的所有组织中都有高表达，表明其在Cd的再活化过程中发挥作用。OsNRAMP2表达的变化影响籽粒中Fe、Mn、Zn和Cu的含量，也影响水稻生长。系统发育分析表明，OsNRAMP 2单倍型在粳稻和印度粳稻中的分布存在差异。在OsNRAMP2的4种单倍型中，第1外显子插入6个碱基的Hap 1的籽粒镉含量比其它3种单倍型低45.3%。水稻3K全基因组测序结果表明，除极少数籼稻材料（4.44%）外，几乎所有粳稻材料（99.3%）都携带OsNRAMP 2的Hap 1。本研究揭示了水稻籽粒镉积累的分子机理，为水稻低镉育种提供了新的研究方向。
Cadmium (Cd) accumulation in rice grain is of health concern. Identifying genes involved in grain Cd accumulation and performing molecular breeding may reduce it. In this study, knockout ofOsNRAMP2, a member of theNRAMPfamily, reduced grain Cd concentrations by more than 38%, and overexpressingOsNRAMP2increased grain Cd concentrations by more than 50%. Physiological experiments showed thatOsNRAMP2facilitated Cd translocation from root to shoot by positively regulating Cd efflux from the vacuoles. At filling stage,OsNRAMP2was highly expressed in all tissues except for husk, suggesting its role in Cd remobilization. Changes inOsNRAMP2expression affected the concentrations of Fe, Mn, Zn, and Cu in grain and also affected rice growth. Phylogenetic analysis showed that the distribution ofOsNRAMP2haplotypes betweenjaponicaandindicawas different. Among the four haplotypes ofOsNRAMP2, Hap 1, with a 6-bp nucleotide insertion in exon 1, had grain Cd concentration at least 45.3% lower than any of the other three haplotypes. Almost all (99.3%)japonicaaccessions but rareindicaaccessions (4.44%) from the 3K sequenced rice genomes carry Hap 1 ofOsNRAMP2. Our study sheds light on the molecular mechanism of grain Cd accumulation and provides a promising target for low-Cd rice breeding.
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影响因子：
4.9
作者：
Uraguchi S;Tanaka N;Hofmann C;Abiko K;Ohkama-Ohtsu N;Weber M;Kamiya T;Sone Y;Nakamura R;Takanezawa Y;Kiyono M;Fujiwara T;Clemens S
通讯作者：
Clemens S
DOI：
10.1073/pnas.95.15.9025
发表时间：
1998-07-21
影响因子：
11.1
作者：
Lu, JMY;Bush, DR
通讯作者：
Bush, DR
影响因子：
--
作者：
Yong-feng Yan;Doug-Hwan Choi;Do-Soon Kim;Byun-Woo Lee
通讯作者：
Yong-feng Yan;Doug-Hwan Choi;Do-Soon Kim;Byun-Woo Lee
影响因子：
3.7
作者：
Yan Y;Zhou YQ;Liang CH
通讯作者：
Liang CH
影响因子：
2
作者：
Nakanishi, Hiromi;Ogawa, Ippei;Nishizawa, Naoko K.
通讯作者：
Nishizawa, Naoko K.