Simulated Microgravity Affects Growth of Escherichia coli and Recombinant β-d-Glucuronidase Production
Simulated Microgravity Affects Growth of Escherichia coli and Recombinant β-d-Glucuronidase Production
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DOI：
10.1007/s12010-009-8836-0
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发表时间：
2010-10
影响因子：
3
通讯作者：
L. Xiang;F. Qi;Dazhang Dai;Chun Xing Li;Y. Jiang
中科院分区：
文献类型：
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作者：
L. Xiang;F. Qi;Dazhang Dai;Chun Xing Li;Y. Jiang
模拟微重力(SMG)对细菌的影响已经从多个方面进行了研究。然而，有关SMG中细菌表达的重组蛋白的生产方面的报道很少。在本研究中，E。研究了pET-28a(+)-pGUS在模拟低剪切力的双轴回转器中转化的大肠杆菌BL21(DE3)细胞及其重组β-d-葡萄糖醛酸苷酶的表达。结果表明，在19℃、28℃和37℃下，SMG的细胞干重分别比NG高16.47%、38.06%和28.79%，重组pGUS的表达效率分别比NG高18.33%、19.36%和33.42%(P<； 0.05)。
Effects of simulated microgravity (SMG) on bacteria have been studied in various aspects. However, few reports are available about production of recombinant protein expressed by bacteria in SMG. In this study growth ofE. coliBL21 (DE3) cells transformed with pET-28a (+)-pgus in double-axis clinostat that could model low shear SMG environment and the recombinant β-d-glucuronidase (PGUS) expression have been investigated. Results showed that the cell dry weights in SMG were 16.47%, 38.06%, and 28.79% more than normal gravity (NG) control, and the efficiency of the recombinant PGUS expression in SMG were 18.33%, 19.36%, and 33.42% higher than that in NG at 19 °C, 28 °C, and 37 °C, respectively (P< 0.05).