Effect of Combined Traumatic Impact and Radial Transection of Medial Meniscus on Knee Articular Cartilage in a Rabbit In Vivo Model
Effect of Combined Traumatic Impact and Radial Transection of Medial Meniscus on Knee Articular Cartilage in a Rabbit In Vivo Model
复制标题
DOI：
10.1016/j.arthro.2012.03.015
复制
发表时间：
2012-10-01
影响因子：
4.7
通讯作者：
Silva, Matthew J.
中科院分区：
文献类型：
--
作者：
Brophy, Robert H.;Martinez, Mario;Silva, Matthew J.
目的：本研究的目的是验证联合手术和创伤性撞击与单纯手术相比加速膝关节软骨早期退变的假设。研究方法：将先前发表的体内兔软骨撞击模型与内侧半月板后角的径向横断相结合，而不是单独进行半月板横断。术后3个月处死家兔。在股骨后髁（PFC）和股骨远端髁（DFC）的撞击部位对手术膝关节和对侧对照膝关节的关节软骨蛋白多糖深度和糖胺聚糖（GAG）分数进行定量组织学分析。结果如下：对于任何测量值，接受单独椎间盘切除术的膝关节的关节软骨与对侧对照膝关节没有显著差异。与对侧对照组相比，联合损伤组膝关节PFC处的GAG分数较低（P = .03），PFC（P = .02）和DFC（P = .04）处的蛋白多糖丢失深度较大。与单纯椎间盘切除术相比，联合损伤膝关节在DFC处有更深的蛋白多糖丢失（P = 0.005）。结论：根据早期使用GAG分数和蛋白多糖深度的结果，联合创伤性撞击和椎间盘切除术比单独椎间盘切除术对关节软骨的损伤更大。
Purpose: The purpose of this study was to test the hypothesis that combined meniscectomy and traumatic impact accelerate early degeneration of articular cartilage in the knee versus meniscectomy alone. Methods: A previously published in vivo rabbit cartilage impact model was used combined with radial transection of the medial meniscus posterior horn versus meniscal transection alone. Rabbits were killed 3 months after surgery. Quantitative histologic analysis of the articular cartilage proteoglycan depth and glycosaminoglycan (GAG) fraction was performed at the site of impact on the posterior femoral condyle (PFC) and at the distal femoral condyle (DFC) overlying the meniscectomy in the surgical knee and the contralateral control knee. Results: The articular cartilage in the knees that underwent isolated meniscectomy did not differ significantly from the contralateral control knees for any measured value. The knees with a combined insult had a lower GAG fraction (P = .03) at the PFC and a greater depth of proteoglycan loss at both the PFC (P = .02) and the DFC (P = .04) versus contralateral controls. Compared with meniscectomy alone, the combined-insult knees had a greater depth of proteoglycan loss at the DFC (P = .005). Conclusions: On the basis of early results using GAG fraction and proteoglycan depth, combined traumatic impact and meniscectomy are more damaging to articular cartilage than meniscectomy alone.