Improved Electrochemical Microsensor for the Real-Time Simultaneous Analysis of Endogenous Nitric Oxide and Carbon Monoxide Generation
Improved Electrochemical Microsensor for the Real-Time Simultaneous Analysis of Endogenous Nitric Oxide and Carbon Monoxide Generation
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DOI：
10.1021/ac2031628
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发表时间：
2012-02-07
影响因子：
7.4
通讯作者：
Lee, Youngmi
中科院分区：
文献类型：
--
作者：
Park, Sarah S.;Kim, Jiyeon;Lee, Youngmi
一种安培型双NO/CO微传感器的基础上，工作电极，包括双铂微盘（每个直径，76 μ m）和Ag/AgCl参比电极覆盖的气体渗透膜。其中一个Pt盘依次电沉积有Pt和Sn;另一个Pt盘沉积有Pt-Fe（III）氧化物纳米复合材料。第一个显示活性的NO和CO的氧化;第二个显示活性仅为NO氧化。在CO和NO的共同存在下，在每个电极上测得的电流，分别代表CO和NO的浓度。该传感器在监测器官组织至少2.5小时和高选择性NO超过CO在Pt Fe（III）氧化物工作电极显示出高稳定性。实时耦合动态变化的NO和CO产生的活C57小鼠肾脏同时和定量响应NO合成酶抑制剂（N-G-硝基-L-精氨酸甲酯），为第一次。CO被发现增加和NO减少后，添加的抑制剂，这表明这些内源性气体之间可能的相互作用。
An amperometric dual NO/CO microsensor was developed on the basis of a working electrode incorporating dual Pt microdisks (each diameter, 76 pm) and a Ag/AgCl reference electrode covered with a gas permeable membrane. One of the Pt disks was sequentially electrodeposited with Pt and Sn; the other Pt disk was deposited with Pt-Fe(III) oxide nanocomposites. The first showed activity for the oxidation of both NO and CO; the second showed activity only for NO oxidation. In the copresence of NO and CO, the currents measured at each electrode, respectively, represented the concentrations of CO and NO. The sensor showed high stability during the monitoring of organ tissue for at least 2.5 h and high selectivity to NO over CO at the Pt Fe(III) oxide working electrode. Real-time coupled dynamic changes of NO and CO generated by a living C57 mouse kidney were monitored simultaneously and quantitatively in response to a NO synthase inhibitor (N-G-nitro-L-arginine methyl ester), for the first time. CO was found to increase and NO decreased upon addition of the inhibitor, suggesting a possible reciprocal interaction between these endogenous gases.