FGF5 AS A REGULATOR OF THE HAIR-GROWTH CYCLE - EVIDENCE FROM TARGETED AND SPONTANEOUS MUTATIONS
FGF5 AS A REGULATOR OF THE HAIR-GROWTH CYCLE - EVIDENCE FROM TARGETED AND SPONTANEOUS MUTATIONS
复制标题
DOI：
10.1016/0092-8674(94)90276-3
复制
发表时间：
1994-09-23
期刊：
影响因子：
64.5
通讯作者：
MARTIN, GR
中科院分区：
文献类型：
--
作者：
HEBERT, JM;ROSENQUIST, T;MARTIN, GR
成纤维细胞生长因子5（FGF5）是一种分泌性信号蛋白。通过基因靶向胚胎干细胞产生的Fgf5基因（fgf5（neo））的预测无效等位基因纯合子小鼠具有异常长的毛发。这种表型似乎与自发突变安哥拉（go）的纯合子小鼠相同。fgf5（neo）和go突变不能互补，并且Fgf5的外显子1在go纯合子的DNA中缺失，表明go是Fgf5的突变等位基因。在野生型小鼠的毛囊中检测到Fgf5的表达，并在毛发生长周期的生长期VI阶段定位于外根鞘。这些发现提供了FGF5作为毛发伸长抑制剂的证据，从而鉴定了一种分子，其正常功能显然是通过毛发生长周期调节毛囊进展中的一个步骤。
Fibroblast growth factor 5 (FGF5) is a secreted signaling protein. Mice homozygous for a predicted null allele of the Fgf5 gene, fgf5(neo), produced by gene targeting in embryonic stem cells, have abnormally long hair. This phenotype appears identical to that of mice homozygous far the spontaneous mutation angora (go). The fgf5(neo) and go mutations fail to complement one another, and exon 1 of Fgf5 is deleted in DNA from go homozygotes, demonstrating that go is a mutant allele of Fgf5. Expression of Fgf5 is detected in hair follicles from wild-type mice and is localized to the outer root sheath during the anagen VI phase of the hair growth cycle. These findings provide evidence that FGF5 functions as an inhibitor of hair elongation, thus identifying a molecule whose normal function is apparently to regulate one step in the progression of the follicle through the hair growth cycle.