REGULATION OF LEYDIG-CELL FUNCTION IN PRIMARY CULTURE BY INHIBIN AND ACTIVIN
REGULATION OF LEYDIG-CELL FUNCTION IN PRIMARY CULTURE BY INHIBIN AND ACTIVIN
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DOI：
10.1210/endo-125-4-2134
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发表时间：
1989-10-01
期刊：
影响因子：
4.8
通讯作者：
BARDIN, CW
中科院分区：
文献类型：
--
作者：
LIN, T;CALKINS, JH;BARDIN, CW
抑制素和激活素分别是选择性抑制和刺激FSH释放的性腺糖蛋白。之前我们报道过转化生长因子。抑制hcg刺激的成熟间质细胞中睾酮的形成。在本研究中，我们评估了转化生长因子- β的其他成员的作用。抑制素和激活素对间质细胞功能的影响。我们发现激活素（0.1-10 ng/ml）对基础睾酮的形成没有影响，但以剂量依赖的方式抑制hcg刺激的睾酮形成。激活素（10 ng/ml）对hcg刺激的睾酮形成的抑制作用为42%。激活素也抑制hcg刺激的cAMP形成。激活素（5 ng/ml）存在时，福斯克林(10 .mu。M)-和8-溴camp （0.1 mM）诱导的睾酮形成减少了约三分之一。孕烯醇酮和孕酮向睾酮的转化也被激活素阻断。有趣的是，[125I]hCG结合间质细胞和福斯克林诱导的cAMP形成不受激活素的影响。与激活素相比，抑制素（0.1-10 ng/ml）在任何浓度下都对hcg诱导的睾酮形成没有影响。然而，激活素对间质细胞功能的抑制作用被同时加入抑制素逆转。我们的研究结果表明，激活素可以抑制正常成熟大鼠的间质细胞形成睾酮。类固醇生成途径的多个步骤受到睾酮的影响。单独抑制素没有作用，但可以逆转激活素的抑制作用。
Inhibin and activin are gonadal glycoproteins that selectively inhibit and stimulate FSH release, respectively. Previously we have reported that transforming growth factor-.beta. inhibited hCG-stimulated testosterone formation in mature Leydig cells. In the present study we evaluated the effects of other members of the transforming growth factor-.beta. family, inhibin and activin, on Leydig cell function. We found that activin (0.1-10 ng/ml) had no effect on basal testosterone formation, but inhibited hCG-stimulated testosterone formation to a dose-dependent manner. Activin (10 ng/ml) inhibited hCG-stimulated testosterone formation by 42%. Activin also inhibited hCG-stimulated cAMP formation. In the presence of activin (5 ng/ml), forskolin (10 .mu.M)- and 8-bromo-cAMP (0.1 mM)-induced testosterone formation were reduced about one third. Conversions of pregnenolone and progesterone to testosterone were also blocked by activin. Interestingly, [125I]hCG binding to Leydig cells and forskolin-induced cAMP formation were not affected by the addition of activin. In contrast to activin, inhibin (0.1-10 ng/ml) had no effect on hCG-induced testosterone formation at any concentration used. However, the inhibitory effects of activin on Leydig cell function were reversed by the concomitant addition of inhibin. Our results suggest that activin inhibits testosterone formation by the Leydig cells derived from normal mature rats. Multiple steps of the steroidogenic pathway are affected by testosterone. Inhibin alone has no effect, but reverses the inhibitory action of activin.