Transient or occult HIV-1 infection in high-risk adults.
Transient or occult HIV-1 infection in high-risk adults.
复制标题
高危成人短暂或隐匿性 HIV-1 感染。
DOI：
10.1097/00002030-200106150-00013
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Cloyd,MW
中科院分区：
文献类型：
--
作者：
Sahu,GK;Chen,JJ;Huang,JC;Ramsey,KM;Cloyd,MW
Infection of humans with HIV-1 results in a persistent, and usually progressive, infection, resulting in the loss of T helper lymphocytes and immunodeficiency. The diagnosis of HIV-1 infection has been based upon the detection of serum anti-HIV-1 antibodies by commercial enzyme immunoassays (EIA) employing denatured HIV antigens, with confirmation by Western blot or fixed-cell immunofluorescence. However, the transient appearance of either anti-HIV-1 antibodies in sera or polymerase chain reaction (PCR)-amplifiable HIV-1 DNA in peripheral blood mononuclear cells (PBMC), or both, has been described in a small number of adults at risk of HIV-1 infection [1]. Although conversion from HIV-1 antibody-positive to negative status is the norm for infants born to infected mothers once maternal IgG in the newborns has cleared, virological data based on HIV-1-DNA amplification by PCR in infant blood specimens at early and later time-points have indicated that clearance of HIV-1 may occur in some infected infants [2]. However, a recent analysis of HIV-1 sequences in some of those infants and their mothers [3] raised doubts about the suspected cases of transient HIV infection. The question thus remains as to whether transient HIV infection occurs.During a prospective study of seronegative sexual partners of known HIV-1-infected patients [4], we identified two apparently transiently infected individuals (nos. 1 and 2) using a live-cell immunofluorescence assay (IFA) as a test for serum anti-HIV-1 antibodies, HIV-1 DNA amplification by PCR, and HIV-1 culture from the PBMC of the subjects (Table 1). These two were ‘early-infected’individuals previously reported (identified by their blood sample codes as R6 and R78) who possessed serum anti-HIV-1 antibodies that reacted with native HIV-1 antigens in live-cell IFA, but did not react in the standard Food and Drug Administration-approved denatured-antigen EIA or Western blot. These antibodies were later shown to react to conformational epitopes of HIV-1 Env (gp160) and Gag (p55) precursor proteins, and appear to be the first antibodies induced after HIV infection (submitted for publication).
