A physiological and proteomic study of poplar leaves during ozone exposure combined with mild drought
A physiological and proteomic study of poplar leaves during ozone exposure combined with mild drought
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DOI：
10.1002/pmic.201200193
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发表时间：
2013-05-01
期刊：
影响因子：
3.4
通讯作者：
Renaut, Jenny
中科院分区：
文献类型：
--
作者：
Bohler, Sacha;Sergeant, Kjell;Renaut, Jenny
在干旱期间出现高臭氧浓度是常见的，因为它们部分是由相同的气象现象引起的。结果表明，轻度干旱可以通过气孔关闭，阻止臭氧进入叶片来保护植物免受臭氧的伤害。本实验试图创建一个在臭氧，轻度干旱和基于使用2D-DiGE来比较所涉及的蛋白质和一些支持分析的组合处理的基础上的细胞过程的变化的概述。形态症状在联合处理时最严重，表明严重的应激，但联合处理的蛋白质差异丰富少于单独臭氧处理。联合处理的气孔导度略有降低。碳代谢的变化表明代谢改变以适应保护措施，而参与氧化还原保护的蛋白质丰度的变化表明氧化应激的存在。这项研究确定了一组在臭氧和干旱胁迫下发生类似变化的蛋白质，这表明植物在暴露于这些胁迫下的分子反应中存在串扰。只有当植物暴露在特定条件下时，其他关键蛋白质的丰度才会发生变化。总的来说，这表明了对不同条件的普遍和专门反应的共存。
The occurrence of high-ozone concentrations during drought episodes is common considering that they are partially caused by the same meteorological phenomena. It was suggested that mild drought could protect plants against ozone-induced damage by causing the closure of stomata and preventing the entry of ozone into the leaves. The present experiment attempts to create an overview of the changes in cellular processes in response to ozone, mild drought and a combined treatment based on the use of 2D-DiGE to compare the involved proteins, and a number of supporting analyses. Morphological symptoms were worst in the combined treatment, indicating a severe stress, but fewer proteins were differentially abundant in the combined treatment than for ozone alone. Stomatal conductance was slightly lowered in the combined treatment. Shifts in carbon metabolism indicated that the metabolism changed to accommodate for protective measures and changes in the abundance of proteins involved in redox protection indicated the presence of an oxidative stress. This study allowed identifying a set of proteins that changed similarly during ozone and drought stress, indicative of crosstalk in the molecular response of plants exposed to these stresses. The abundance of other key proteins changed only when the plants are exposed to specific conditions. Together this indicates the coexistence of generalized and specialized responses to different conditions.