Reduced gene expression of E-cadherin and associated catenins in human cervical carcinoma cell lines
Reduced gene expression of E-cadherin and associated catenins in human cervical carcinoma cell lines
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DOI：
10.1016/s0304-3835(97)00308-x
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发表时间：
1997-12-09
期刊：
影响因子：
9.7
通讯作者：
Schwarz, E
中科院分区：
文献类型：
--
作者：
Denk, C;Hulsken, J;Schwarz, E
在人类癌症中，E-钙粘蛋白介导的细胞间粘附经常丢失或受到干扰。为了实现正常的粘附功能，E-钙粘蛋白必须与外周细胞质连环蛋白形成复合物，外周细胞质连环蛋白是多功能蛋白，也参与信号转导和生长调节。我们分析了源自人宫颈癌的细胞系中编码α-连环蛋白、β-连环蛋白和斑珠蛋白的基因的表达水平与E-钙粘蛋白表达水平的相关性。检测到斑珠蛋白的 mRNA 和蛋白质水平降低，而 α- 和 β-连环蛋白仅显示蛋白质（而非 mRNA）水平降低。连环蛋白基因表达的改变通常与 E-钙粘蛋白的缺失或减少有关。研究结果表明，连环蛋白基因表达的减少可能有助于宫颈癌的发展。 (C) 1997 爱思唯尔科学爱尔兰有限公司
Cell-cell adhesion mediated by E-cadherin is often lost or disturbed in human carcinomas. For regular adhesive function, E-cadherin has to form complexes with peripheral cytoplasmic catenins which are multifunctional proteins that are also involved in signal transduction and growth regulation. We have analyzed the expression levels of the genes encoding alpha-catenin, beta-catenin and plakoglobin in correlation to the E-cadherin expression levels in cell lines derived from human cervical carcinomas. Reduced mRNA and protein levels were detected for plakoglobin, whereas alpha- and beta-catenin showed only reduced protein (but not mRNA) levels. The alterations in catenin gene expression were often associated with absent or reduced E-cadherin. The findings indicate that a reduction of catenin gene expression may contribute to the development of cervical carcinomas. (C) 1997 Elsevier Science Ireland Ltd.