Cell-microenvironment interactions and architectures in microvascular systems.
Cell-microenvironment interactions and architectures in microvascular systems.
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DOI：
10.1016/j.biotechadv.2016.07.002
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发表时间：
2016-11-01
影响因子：
16
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Bersini, Simone;Yazdi, Iman K.;Talo, Giuseppe;Shin, Su Ryon;Moretti, Matteo;Khademhosseini, Ali
关键词：
在过去的十年中，在设计和优化新型生物材料和微制造技术以产生血管化组织方面取得了重大进展。新型微流体系统促进了体外模型的开发和优化，用于探索微血管环境内发生的复杂病理生理现象。到目前为止，这些模型中的大多数都集中在日益复杂的系统的工程，而不是分析驱动微血管网络形态发生和重塑的分子和细胞机制。事实上，内皮细胞（EC）、支持壁细胞和器官特异性细胞之间以及EC与细胞外基质之间的相互作用是血管化的关键驱动力。本文综述了材料科学、微工程和血管生物学在体外微血管系统开发中的应用。目前正在应用的各种方法来研究细胞-细胞/细胞-基质相互作用，以及生物化学/生物物理线索促进血管化及其对微血管网络形成的影响，将被确定和讨论。最后，本文将探讨微血管系统的体外应用，移植血管化组织的体内整合，以及血管化和控制工程组织内的微循环系统，特别是微制造方法的重要挑战。现有的模型和更复杂的模型可能会进一步加深我们对血管网络生长、稳定和重塑的关键要素的理解，将基本研究原理转化为功能性血管化组织构建，用于再生医学应用、药物筛选和疾病模型。
In the past decade, significant advances have been made in the design and optimization of novel biomaterials and microfabrication techniques to generate vascularized tissues. Novel microfluidic systems have facilitated the development and optimization of in vitro models for exploring the complex pathophysiological phenomena that occur inside a microvascular environment. To date, most of these models have focused on engineering of increasingly complex systems, rather than analyzing the molecular and cellular mechanisms that drive microvascular network morphogenesis and remodeling. In fact, mutual interactions among endothelial cells (ECs), supporting mural cells and organ-specific cells, as well as between ECs and the extracellular matrix, are key driving forces for vascularization. This review focuses on the integration of materials science, microengineering and vascular biology for the development of in vitro microvascular systems. Various approaches currently being applied to study cell-cell/cell-matrix interactions, as well as biochemical/biophysical cues promoting vascularization and their impact on microvascular network formation, will be identified and discussed. Finally, this review will explore in vitro applications of microvascular systems, in vivo integration of transplanted vascularized tissues, and the important challenges for vascularization and controlling the microcirculatory system within the engineered tissues, especially for microfabrication approaches. It is likely that existing models and more complex models will further our understanding of the key elements of vascular network growth, stabilization and remodeling to translate basic research principles into functional, vascularized tissue constructs for regenerative medicine applications, drug screening and disease models.
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影响因子：
14
作者：
Benton, Julie A.;Fairbanks, Benjamin D.;Anseth, Kristi S.
通讯作者：
Anseth, Kristi S.
影响因子：
3
作者：
Buchanan, Cara F.;Voigt, Elizabeth E.;Rylander, Marissa Nichole
通讯作者：
Rylander, Marissa Nichole
分子机制和血管生成的临床应用。
影响因子：
64.8
作者：
Carmeliet, Peter;Jain, Rakesh K.
通讯作者：
Jain, Rakesh K.
影响因子：
3.7
作者：
Carrion, Bita;Kong, Yen P.;Kaigler, Darnell;Putnam, Andrew J.
通讯作者：
Putnam, Andrew J.
影响因子：
3.1
作者：
AUSPRUNK, DH;FOLKMAN, J
通讯作者：
FOLKMAN, J