Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells
Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells
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DOI：
10.1016/j.febslet.2006.09.053
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发表时间：
2006-10-30
期刊：
影响因子：
3.5
通讯作者：
Kim, Jong-Hoon
中科院分区：
文献类型：
--
作者：
Kim, Byung-Kak;Kim, Sung-Eun;Kim, Jong-Hoon
血管内皮生长因子（VEGF）是一种对血管内皮细胞有强大促有丝分裂作用的因子，被认为是一种参与神经发生以及血管生成的调节因子。在此，我们利用人胚胎干细胞（hESCs）直接检测了VEGF对神经外胚层分化的影响。在拟胚体（EBs）的胚层形成过程中，VEGF处理上调了神经外胚层基因（Sox1和巢蛋白）的表达，并有效增加了同时表达Pax6和巢蛋白的神经花环的数量。由VEGF处理的拟胚体产生的神经祖细胞进一步分化为在终末分化时呈现出在多巴胺能神经元发育过程中所观察到的相似基因表达模式的细胞。这些结果支持了VEGF对人胚胎干细胞分化的神经发生作用。（c）2006欧洲生物化学学会联合会。由爱思唯尔出版集团出版。保留所有权利。
Vascular endothelial growth factor (VEGF), a potent mitogen for vascular endothelial cells, has been suggested as a modulator that is involved in neurogenesis as well as angiogenesis. Here, we directly examined the effect of VEGF on neuroectodermal differentiation using human embryonic stem cells (hESCs). VEGF treatment upregulated the expression of neuroectodermal genes (SoxI and Nestin) during germ layer formation in embryoid bodies (EBs) and efficiently increased the number of neural rosettes expressing both Pax6 and Nestin. The neural progenitors generated from VEGF-treated EBs further differentiated into cells that showed a similar pattern of gene expression observed in the development of dopaminergic neurons upon terminal differentiation. These results support the neurogenic effect of VEGF on hESC differentiation. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.