Comparative in vitro cytotoxicity of modified deoxynivalenol on porcine intestinal epithelial cells
Comparative in vitro cytotoxicity of modified deoxynivalenol on porcine intestinal epithelial cells
复制标题
DOI：
10.1016/j.fct.2016.06.012
复制
发表时间：
2016-09-01
影响因子：
4.3
通讯作者：
Croubels, Siska
中科院分区：
文献类型：
--
作者：
Broekaert, Nathan;Devreese, Mathias;Croubels, Siska
通过饲料和食物摄入霉菌毒素脱氧雪腐镰刀菌烯醇 (DON) 后，胃肠道是第一个目标。已知脱氧雪腐镰刀菌烯醇会影响动物和人类肠上皮细胞的增殖和活力。除 DON 外，饲料和食品中还经常被修饰形式的 DON 共同污染，例如 3-乙酰脱氧雪腐镰刀菌烯醇 (3ADON)、15-乙酰基-脱氧雪腐镰刀菌烯醇 (15ADON) 和脱氧雪腐镰刀菌烯醇-3-β-D-葡萄糖苷 (DON3G)。本研究的目的是通过流式细胞术确定这些修饰形式对分化和增殖的猪肠上皮细胞的体外内在细胞毒性。通过使用膜联蛋白-V-异硫氰酸荧光素 (FITC) 和碘化丙啶 (PI) 双重染色来评估细胞死亡，从而区分活细胞 (FITC-/PI-)、凋亡细胞 (FITC+/PI-) 和坏死细胞 (FITC-F/PI+)。根据所提供的体外试点研究的数据，得出的结论是，对增殖细胞的细胞毒性可分级如下： DON3G
The gastrointestinal tract is the first target after ingestion of the mycotoxin deoxynivalenol (DON) via feed and food. Deoxynivalenol is known to affect the proliferation and viability of animal and human intestinal epithelial cells. In addition to DON, feed and food is often co-contaminated with modified forms of DON, such as 3-acetyldeoxynivalenol (3ADON), 15-acetyl-deoxynivalenol (15ADON) and deoxynivalenol-3-beta-D-glucoside (DON3G). The goal of this study was to determine the in vitro intrinsic cytotoxicity of these modified forms towards differentiated and proliferative porcine intestinal epithelial cells by means of flow cytometry. Cell death was assessed by dual staining with Annexin-V-fluorescein isothiocyanate (FITC) and propidium iodide (PI), which allows the discrimination of viable (FITC-/PI-), apoptotic (FITC+/PI-) and necrotic cells (FITC-F/PI+). Based on the data from the presented pilot in vitro study, it is concluded that cytotoxicity for proliferative cells can be ranked as follows: DON3G