Programmed Vesicle Fusion Triggers Gene Expression
Programmed Vesicle Fusion Triggers Gene Expression
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DOI：
10.1021/la202648h
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发表时间：
2011-11-01
期刊：
影响因子：
3.9
通讯作者：
Yomo, Tetsuya
中科院分区：
文献类型：
--
作者：
Caschera, Filippo;Sunami, Takeshi;Yomo, Tetsuya
磷脂囊泡的膜特性可以调节和启动包封的生化反应和网络。我们提出的证据，抑制和激活反应封装在囊泡外源添加带电两亲体。虽然阳离子两亲体的掺入具有抑制作用，但额外的阴离子两亲体的补充使反应恢复活力。我们演示了β -葡萄糖醛酸酶的简单水解反应以及该酶在DNA模板上的体外基因表达。此外，我们发现两个囊泡群分别用正、负两亲体装饰，可以选择性地融合，提供喂养成分，以启动封装反应。该机制可能是动态人工细胞模型中调控和维持代谢的基本而有效的手段之一。
The membrane properties of phospholipid vesicles can be manipulated to both regulate and initiate encapsulated biochemical reactions and networks. We present evidence for the inhibition and activation of reactions encapsulated in vesicles by the exogenous addition of charged amphiphiles. While the incorporation of cationic amphiphile exerts an inhibitory effect, complementation of additional anionic amphiphiles revitalize the reaction. We demonstrated both the simple hydrolysis reaction of beta-glucuronidase and the in vitro gene expression of this enzyme from a DNA template. Furthermore, we show that two vesicle populations decorated separately with positive and negative amphiphiles can fuse selectively to supply feeding components to initiate encapsulated reactions. This mechanism could be one of the rudimentary but effective means to regulate and maintain metabolism in dynamic artificial cell models.