MYOSIN TYPES IN HUMAN SKELETAL-MUSCLE FIBERS
MYOSIN TYPES IN HUMAN SKELETAL-MUSCLE FIBERS
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DOI：
10.1007/bf00493174
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
JENNY, E
中科院分区：
文献类型：
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作者：
BILLETER, R;WEBER, H;JENNY, E
通过将纤维分型的酶组织化学与慢速和快速肌球蛋白的免疫组织化学相结合，在人类骨骼肌中寻找纤维类型和肌球蛋白类型之间的相关性。在不同的 pH 值下预孵育后，通过对肌原纤维 ATP 酶进行染色来进行纤维分型。使用与人肌球蛋白发生交叉反应的类型特异性抗兔肌球蛋白抗体来区分肌球蛋白类型，并通过蛋白 A-过氧化物酶方法进行可视化。 I 型纤维仅含有慢肌球蛋白，IIA 型和 IIB 型纤维仅含有快肌球蛋白，IIC 型纤维均含有不同比例的肌球蛋白。当对训练有素的运动员的肌肉活检进行研究时，观察到基本上相同的染色模式。很少出现的具有高糖酵解活性的 I 型纤维含有额外的少量快肌球蛋白，偶尔的 IIA 型纤维含有少量的慢肌球蛋白。基于对慢速和快速肌球蛋白共存的各种纤维类型的观察，提出了涵盖所有纤维类型的动态纤维连续体。
By combining enzyme histochemistry for fiber typing with immunohistochemistry for slow and fast myosin a correlation between fiber type and myosin type was sought in human skeletal muscle. Fiber typing was done by staining for myofibrillar ATPases after preincubation at discriminating pH values. Myosin types were discriminated using type specific anti-rabbit myosin antibodies shown to cross-react with human myosin and were visualized by a protein A-peroxidase method. Type I fibers contain slow myosin only, type IIA and IIB fibers fast myosin only and type IIC fibers both myosins in various proportions. When muscle biopsies from well-trained athletes were investigated, essentially the same staining pattern was observed. Rarely occurring type I fibers with high glycolytic activity were detected containing additional small amounts of fast myosin and occasional type IIA fibers had small amounts of slow myosin. Based on the observation of various fiber types in which slow and fast myosin coexist a dynamic continuum of fibers encompassing all fiber types is proposed.