A HAPLOID AND A DIPLOID-CELL CYCLE COEXIST IN AN IN-VITRO IMMORTALIZED SPERMATOGENIC CELL-LINE
A HAPLOID AND A DIPLOID-CELL CYCLE COEXIST IN AN IN-VITRO IMMORTALIZED SPERMATOGENIC CELL-LINE
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DOI：
10.1002/dvg.1020160205
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发表时间：
1995-01-01
期刊：
影响因子：
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通讯作者：
MILLAN, JL
中科院分区：
文献类型：
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作者：
HOFMANN, MC;ABRAMIAN, D;MILLAN, JL
我们最近建立了一种条件永生生殖细胞系[GC-2spd(ts)]，在37℃和32℃的允许温度下，能够在体外进行减数分裂并形成圆形精子[Hofmann等人，（1994）：Proc Nail Acad Sci USA 91:5533-5537]。在本报告中，我们提供的数据表明，GC-2spd（ts）细胞系由两个细胞群组成，分别经历单倍体（n/2n）细胞周期和二倍体（2n/4n）细胞周期。含有2n DNA的细胞经荧光激活细胞分选后，能够重建完整的n/2n/4n DNA细胞群，这表明它们能够进行减数分裂并形成单倍体精子，或通过二倍体细胞周期继续自我复制。GC-2spd（ts）细胞系为研究细胞有丝分裂自我更新和减数分裂之间的分子机制提供了有价值的工具。(C) 1995 Wiiey-Liss, Inc。
We have recently established a conditionally immortalized germ cell line [GC-2spd(ts)] that, at the permissive temperatures of 37 degrees C and 32 degrees C, is able to undergo meiosis in vitro and form round spermatids [Hofmann ef al., (1994): Proc Nail Acad Sci USA 91:5533-5537]. In this report, we provide data that indicate that the GC-2spd(ts) cell line consists of two cell populations undergoing a haploid (n/2n) cell cycle and a diploid (2n/4n) cell cycle, respectively. The cells containing 2n DNA, when sorted by fluorescence-activated cell sorting, are able to reconstitute the full population of n/2n/4n DNA cells, indicating that they are able to commit to the reductive meiotic division and form haploid spermatids or to continue self-replication through a diploid cell cycle. This GC-2spd(ts) cell line provides a valuable tool to study the molecular mechanisms involved in the cellular decision between self-renewal by mitosis and commitment to meiosis. (C) 1995 Wiiey-Liss, Inc.