A turn-on fluorescent chemosensor based on aggregation-induced emission for cyanide detection and its bioimaging applications
A turn-on fluorescent chemosensor based on aggregation-induced emission for cyanide detection and its bioimaging applications
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基于聚集诱导发射的开启荧光化学传感器用于氰化物检测及其生物成像应用
DOI：
10.1016/j.snb.2019.126645
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发表时间：
2019-10-01
影响因子：
8.4
通讯作者：
Qu, Jinqing
中科院分区：
文献类型：
--
作者：
Deng, Kailun;Wang, Lei;Qu, Jinqing
由于氰离子(CN-)不可避免地被用于各种领域，对人类健康和生态系统有害，因此人们致力于开发用于定性和定量检测氰离子(CN-)的荧光传感器。本文设计并合成了一种具有聚集诱导发射特性的新型荧光探针TCNT。碳纳米管具有较大的斯托克斯位移(约148 nm)和红色荧光发射(596 Nm)，对CN-具有很高的特异性和灵敏度(检测下限为0.35mU·mol·L~(-1))，在水溶液中的量子产率提高了2.53倍。通过H-1核磁共振滴定和密度泛函计算证实了碳纳米管对氰化物的识别机理是由于CN-对乙烯基团的亲核攻击。在实际环境中，装载了碳纳米管的试纸可以方便地检测氰离子。此外，由于TCNT具有良好的生物相容性和较低的细胞毒性，将其应用于激光共聚焦扫描显微镜监测HEG-2细胞中CN-的含量。此外，对小鼠分离器官的荧光成像证明，TCNT能够在活体中实现生物成像。
Numerous efforts are contributed to develop fluorescent sensors for qualitative and quantitative detection of cyanide anion (CN-) as it is inevitably utilized in various fields and detrimental to human health and ecosystem. Herein, a new fluorescent probe TCNT with the feature of aggregation-induced emission was designed and synthesized. Owning a large Stokes shift ((similar to)148 nm) and red fluorescent emission (596 nm), TCNT showed high specific and sensitivity (detection limit equals to 0.35 mu mol L-1) to CN- with a 2.53-fold enhancement of quantum yield in aqueous solution. The recognition mechanism of TCNT sensing cyanide was ascribed to nucleophilic attack of CN- toward vinyl group, which was confirmed by H-1 NMR titration and DFT calculations. Test strips loaded with TCNT demonstrated its convenient detection for cyanide ion in real environment. In addition, because of good biocompatibility and low cytotoxicity, TCNT was applied for monitoring the levels of CN- in HeG-2 cells by confocal laser scanning microscopy. Moreover, fluorescence imaging of isolated organs of mice proved that TCNT is capable of achieving bioimaging in live organisms.