PROMOTION OF THE TRANSLOCATION OF ENTERIC BACTERIA FROM THE GASTROINTESTINAL TRACTS OF MICE BY ORAL TREATMENT WITH PENICILLIN, CLINDAMYCIN, OR METRONIDAZOLE
PROMOTION OF THE TRANSLOCATION OF ENTERIC BACTERIA FROM THE GASTROINTESTINAL TRACTS OF MICE BY ORAL TREATMENT WITH PENICILLIN, CLINDAMYCIN, OR METRONIDAZOLE
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DOI：
10.1128/iai.33.3.854-861.1981
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发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
BERG, RD
中科院分区：
文献类型：
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作者：
BERG, RD
用抗生素口服治疗无特定病原体的小鼠，以确定所导致的正常植物群生态的破坏是否会使某些革兰氏阴性肠道细菌过度繁殖盲肠，从而促进这些细菌从胃肠道移位。小鼠口服青霉素、克林霉素或甲硝唑4天，然后停用抗生素。以不同的时间间隔检测小鼠从胃肠道移位至肠系膜淋巴结的活肠杆菌。青霉素治疗降低了盲肠中的总厌氧菌群体水平平均为1000倍;克林霉素治疗降低了这些厌氧菌水平只有10倍;甲硝唑治疗略有增加厌氧菌水平。青霉素或甲硝唑治疗使盲肠中的肠杆菌群平均增加1000倍;克林霉素治疗使肠杆菌群增加100，000倍。肠杆菌移位至肠系膜淋巴结的高峰发生率在青霉素治疗后平均为100%，克林霉素治疗后为97%，甲硝唑治疗后为62%。因此，用青霉素、克林霉素或甲硝唑口服治疗小鼠仅4天，就破坏了正常的植物群生态，使得革兰氏阴性肠杆菌在盲肠中过度生长，并促进它们移位至肠系膜淋巴结。
Specific pathogen-free mice were treated orally with antibiotics to determine whether the resulting disruption of the normal flora ecology would allow certain gram-negative enteric bacteria to overpopulate the ceca, thereby promoting the translocation of these bacteria from the gastrointestinal tract. The mice were treated orally with penicillin, clindamycin or metronidazole for 4 days, and then the antibiotic was discontinued. The mice were tested at various intervals for viable enteric bacilli translocating from the gastrointestinal tract to the mesenteric lymph nodes. Penicillin treatment decreased the total anaerobe population levels in the ceca an average of 1000-fold; clindamycin treatment decreased these anaerobe levels only 10-fold; metronidazole treatment slightly increased the anaerobe levels. Penicillin or metronidazole treatment increased the enteric bacilli populations in the ceca an average of 1000-fold; clindamycin treatment increased the enteric bacilli populations 100,000-fold. The peak incidence of translocation of the enteric bacilli to the mesenteric lymph nodes averaged 100% after penicillin treatment, 97% after clindamycin treatment and 62% after metronidazole treatment. Thus, oral treatment of mice with penicillin, clindamycin or metronidazole for only 4 days disrupts the normal flora ecology, allowing an overgrowth in the ceca of the gram-negative enteric bacilli and promoting their translocation to the mesenteric lymph nodes.