Recovery of arbutin in high purity from fruit peels of pear (Pyrus pyrifolia Nakai)
Recovery of arbutin in high purity from fruit peels of pear (Pyrus pyrifolia Nakai)
复制标题
DOI：
10.1007/s10068-011-0111-9
复制
发表时间：
2011-06-01
影响因子：
2.9
通讯作者：
Moon, Jae-Hak
中科院分区：
文献类型：
--
作者：
Cho, Jeong-Yong;Park, Keun Young;Moon, Jae-Hak
β-熊果苷是梨果实中含量最丰富的化合物之一，已被广泛用作化妆品中的增白剂。本研究采用一种简便的方法从梨果皮中分离纯化高纯度的熊果苷。Chuhwangshan）开发。在酸性条件下（pH3.0），用80%乙醇提取熊果苷，熊果苷的提取率最高。在水和乙酸乙酯之间的分配中，几乎所有的熊果苷（78%）都在水层中检测到。采用Diaion HP-20柱层析和制备型HPLC对熊果苷进行了纯化和分离。分离的熊果苷代表60%的回收率，纯度超过99%。
beta-Arbutin is one of the most abundant compounds in pear fruit and has been widely used as a whitening agent in cosmetic products. In this study, a simple method for the purification and isolation of arbutin in high purity from the fruit peel of a pear (Pyrus pyrifolia Nakai cv. Chuhwangbae) was developed. The high extraction efficiency of arbutin was accomplished with 80% ethanol under acidic conditions (pH 3.0). In the partition between water and ethyl acetate, almost all of the arbutin (78%) was detected in the water layer. Therefore, arbutin was further purified and isolated from the water layer using Diaion HP-20 column chromatography and preparative HPLC. The isolated arbutin represented a 60% recovery with a purity exceeding 99%.