INDUCTION AND REPAIR OF BENZO[A]PYRENE-DNA ADDUCTS IN C57BL/6 AND BALB/C MICE - ASSOCIATION WITH AGING AND LONGEVITY
INDUCTION AND REPAIR OF BENZO[A]PYRENE-DNA ADDUCTS IN C57BL/6 AND BALB/C MICE - ASSOCIATION WITH AGING AND LONGEVITY
复制标题
DOI：
10.1016/0047-6374(95)01603-w
复制
发表时间：
1995-07-28
影响因子：
5.3
通讯作者：
VIJG, J
中科院分区：
文献类型：
--
作者：
BOERRIGTER, METI;WEI, JY;VIJG, J
本研究采用灵敏的P-32后标记技术，研究了年龄对两个不同寿命品系小鼠（C57 BL/6 ByJ（C57 BL/6）和BALB/cByJ（BALB/c））6个器官中苯并[a]芘（B[a]P）DNA加合物形成和消失的影响。在用50 mg B[a]P/kg体重进行单次腹膜内处理后，主要B[a] P衍生加合物反式-（7 R）-N-2-[10-（10-甲基-2-氧代-2-苯基）-N-[10-（10-甲基-2-氧代-2-苯基）-N-[10-（10-甲基-2-氧代-2-苯基）-N-（10-甲基-2-氧代-2-苯基）-N-[10-（10-甲基-2-氧代-2-苯基）-N-[10-（10-甲基-2-氧代-2-苯基）-N-（10-甲基-2-氧代-2-苯基）-N-[10-（10-甲基-2-氧代-2-苯基）-N-（10-甲基-2-氧代-2-苯基）（7 β，8 α，9 α-三羟基-7，8，9，10）-四氢苯并[a]芘]-基-脱氧鸟苷（BPDE-N-2-dG），似乎是年龄和器官依赖性的;两个小鼠品系之间的相同器官观察到微小差异。年轻小鼠和老年小鼠不同器官中BPDE-N-2-dG的最大形成量相差2-4倍，老年小鼠器官中BPDE-N-2-dG的最大形成量比年轻小鼠低2 - 8倍。BPDE-N-2-dG的去除，治疗后7天，显然是年龄和应变依赖性;观察到的非显着差异，在每个年龄段的菌株内的器官研究。在预期寿命比BALB/c长的年轻C57 BL/6小鼠中，与年轻BALB/c相比，肝脏和心脏中BPDE-N-2-dG的消失率显著更高。与BALB/c小鼠相比，在C57 BL/6小鼠的器官中，在年龄较大时，观察到BPDE-N-2-dG消失率降低的频率更高，且程度相对更大。这些结果进行了讨论，在寿命的差异和病理病变的发病率之间的两个品系的小鼠。
In this study, we employed the sensitive P-32-postlabeling assay to assess the influence of age on the formation and disappearance of benzo[a]pyrene (B[a]P) DNA adducts in six organs of two different mouse strains with different life spans, C57BL/6ByJ (C57BL/6) and BALB/cByJ (BALB/c). Following a single, intraperitoneal treatment with 50 mg B[a]P per kg of bodyweight, maximum formation of the major B[a]P-derived adduct, trans-(7R)-N-2-[10-(7 beta,8 alpha,9 alpha-trihydroxy- 7,8,9,10)-tetrahydrobenzo[a]pyrene]-yl-deoxyguanosine (BPDE-N-2-dG), appeared to be age- and organ-dependent; minor differences were observed for the same organs between the two mouse strains. The maximum formation of BPDE-N-2-dG in the various organs from young and old mice differed by a factor of 2-4 and was two- to eightfold lower in organs from old mice as compared to young mice. The removal of BPDE-N-2-dG, up to 7 days after the treatment, was apparently age- and strain-dependent; non-significant differences were observed for organs within strains at each age studied. In young C57BL/6 mice, which have a greater life expectancy than BALB/c, the rate of disappearance of BPDE-N-2-dG was significantly higher in liver and heart as compared to young BALB/c. At the older age a decrease in the rate of BPDE-N-2-dG disappearance was observed more frequently, and to a relatively greater extent, in organs from C57BL/6 mice as compared to BALB/c mice. These results are discussed in relation to the differences in life spans and the incidence of pathological lesions between the two strains of mice.