A gain-of-function suppressor screen for genes involved in dorsal-ventral boundary formation in the Drosophila wing
A gain-of-function suppressor screen for genes involved in dorsal-ventral boundary formation in the Drosophila wing
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DOI：
10.1534/genetics.107.081869
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发表时间：
2008-01-01
期刊：
影响因子：
3.3
通讯作者：
Milan, Marco
中科院分区：
文献类型：
--
作者：
Bejarano, Fernando;Luque, Carlos M.;Milan, Marco
果蝇翅原基被D细胞中的LTM同源结构域蛋白Apterous的活性细分为背侧（D）和腹侧（V）室。D和V细胞之间的细胞相互作用诱导DV边界处的Notch的激活。刻槽是维持隔室边界和翅原基生长所必需的。Beadex是dLMO的功能获得性等位基因，导致dLMO蛋白水平增加，从而干扰Apterous的活性并导致DV轴形成缺陷。我们进行了功能获得性增强子-启动子（EP）筛选，以寻找Beadex在D细胞中过表达时的抑制子。我们鉴定了53个品系对应35个基因。基因座编码的微小RNA和蛋白质参与染色质组织，转录控制，和囊泡贩运的dLMO活动和DV边界形成的背景下，其特征在于。我们的研究结果表明，与经典的基于功能丧失的筛选相比，在致敏背景中进行功能获得的遗传筛选是鉴定参与发育过程的冗余基因的非常有效的方法。
The Drosophila wing primordium is subdivided into a dorsal (D) and a ventral (V) compartment by the activity of the LTM-homeodomain protein Apterous in D cells. Cell interactions between D and V cells induce the activation of Notch at the DV boundary,. Notch is required for the maintenance of the compartment boundary and the growth of the wing primordium. Beadex, a gain-of function allele of dLMO, results in increased levels of dLMO protein, which interferes with the activity of Apterous and results in defects in DV axis formation. We performed a gain-of-function enhancer-promoter (EP) screen to search for suppressors of Beadex when overexpressed in D cells. We identified 53 lines corresponding to 35 genes. Loci encoding for micro-RNAs and proteins involved in chromatin organization, transcriptional control, and vesicle trafficking were characterized in the context of dLMO activity and DV boundary formation. our results indicate that a gain-of-function genetic screen in a sensitized background, as opposed to classical loss-of-function-based screenings, is a very efficient way to identify redundant genes involved in a developmental process.