Integrin-linked kinase controls vascular wall formation by negatively regulating Rho/ROCK-mediated vascular smooth muscle cell contraction
Integrin-linked kinase controls vascular wall formation by negatively regulating Rho/ROCK-mediated vascular smooth muscle cell contraction
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DOI：
10.1101/gad.535409
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发表时间：
2009-10-01
影响因子：
10.5
通讯作者：
Adams, Ralf H.
中科院分区：
文献类型：
--
作者：
Kogata, Naoko;Tribe, Rachel M.;Adams, Ralf H.
血管平滑肌细胞 (VSMC) 在较大血管周围形成收缩层，这一过程对于形成功能齐全的脉管系统至关重要。在这里，我们发现整合素连接激酶（ILK）是小动脉周围排列的 VSMC 单一层的形成以及小鼠血管收缩的调节所必需的。在不存在 ILK 的情况下，激活的 Rho/ROCK 信号传导会诱导肌球蛋白轻链磷酸化升高，导致体外和体内 VSMC 收缩异常增强。我们的研究结果表明，ILK 是通过负向调节 VSMC 收缩性来调节血管壁形成的关键成分。
Vascular smooth muscle cells (VSMCs) form contractile layers around larger blood vessels in a process that is essential for the formation of a fully functional vasculature. Here, we show that integrin-linked kinase (ILK) is required for the formation of a unitary layer of aligned VSMCs around arterioles and the regulation of blood vessel constriction in mice. In the absence of ILK, activated Rho/ROCK signaling induces the elevated phosphorylation of myosin light chain leading to abnormally enhanced VSMC contraction in vitro and in vivo. Our findings identify ILK as a key component regulating vascular wall formation by negatively modulating VSMC contractility.