Increased adherence of monocytes to fibronectin in bronchiectasis. Regulatory effects of bacterial lipopolysaccharide and role of CD11/CD18 integrins.
Increased adherence of monocytes to fibronectin in bronchiectasis. Regulatory effects of bacterial lipopolysaccharide and role of CD11/CD18 integrins.
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支气管扩张时单核细胞对纤连蛋白的粘附增加。
DOI：
10.1164/ajrccm/145.3.626
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Stockley,RA
中科院分区：
文献类型：
--
作者：
Owen,CA;Campbell,EJ;Hill,SL;Stockley,RA
monoc的合规性：ytesto细胞外基质是肺部感染和炎症过程中单核吞噬细胞积聚的一种载体。我们从炎症性肺疾病（支气管扩张）患者和对照组中获得单核细胞，并比较了它们对纤连蛋白的粘附性。对照组单核细胞的自发粘附率为20 ± 2%，而患者的单核细胞的自发粘附率显著高于对照组，并与气道炎症程度相关：脓痰和粘液痰患者的自发粘附率分别为65±5%和40±8%。内毒素和气道内分泌物可能是引起单核细胞活化的原因，因为：（1）在所有患者中均可检测到内毒素，而在对照组中无内毒素;（2）LPS对正常单核细胞的粘附产生剂量相关的增加（在11191 ml LPS时最大粘附为65±2%）;（3）重组内毒素和LPS对单核细胞的粘附产生累加效应。患者的单核细胞与纤连蛋白的粘附基本上由CD 111 CD 18整合素介导，通过RGD依赖性和RGo非依赖性机制。结果表明，支气管扩张患者肺部感染和炎症灶产生的信号可影响单核细胞的粘附，可能是单核吞噬细胞聚集的决定因素。AM修订版DIS 1992; 145：826-831
Regulatedadherence of monoc: ytesto extracellular matrix Is a pt8l'8qUlsltefor accumulation of mononuclear phagocytes during pulmonary Infection and Inflammation. wehave obtalnad monocytes from patients with an Inflammatory lung dlse_ (bronchiectasis) and from control sub-jects and h_ compared their adherence to flbronectln. Spontaneous adherence of monocytes from the control subjects was20±2%, whereas that of patients' cells was marlciadly higher and correlated with the _rIty of airway Inflammation: 65±5% and 40±8% In patients with purulent and mucoid sputum, respectively. Endotoxin and cytoklnes from arees of airway dl _ are likely to be responslble for the observed monocyte activation, since:(1) endotoxin was detectable In all of the patients but In none of the control subjects;(2) LPS produced a dose-related Incre_ In adherence of normal monocytas in" ifro (maximal 65±2% adherence at 11191ml of LPS);(3) recom· blnant cytoklnes and LPSproduced additive effects on monocyte adherence In" ifro. The adherence of the patients' monocytes to flbronectln was substantially mediated by CD111CD18 Integrlns, via both RGD-dependent and RGo-lndependent mechanisms. Th_ data Indicate that signals arising from foci of Infection and Inflammation can Influence the adherence of monocytes, and they are likely to be determinants of the accumulation of mononuclear phagocytes In the lungs of patients with bronchiectasis. AM REV RESPIR DIS 1992; 145: 826-831