Apoptotic and anti-adhesion effect of ajoene, a garlic derived compound, on the murine melanoma B16F10 cells: possible role of caspase-3 and the alpha(4)beta(1) integrin.
Apoptotic and anti-adhesion effect of ajoene, a garlic derived compound, on the murine melanoma B16F10 cells: possible role of caspase-3 and the alpha(4)beta(1) integrin.
复制标题
阿霍烯（一种大蒜衍生化合物）对小鼠黑色素瘤 B16F10 细胞的凋亡和抗粘附作用：caspase-3 和 alpha(4)beta(1) 整合素的可能作用。
作者：
E. Ledezma;R. Apitz‐Castro;J. Cardier
在这项研究中，我们评估了这样一个假设，即阿焦烯的抗肿瘤活性可能与其诱导肿瘤细胞增殖的作用有关，并与其阻断小鼠黑色素瘤B16 F10细胞中α（4）β（1）整合素表达的能力有关。阿焦烯以剂量依赖性方式诱导B16 F10活力的显著降低（IC（50）=62 μ M）。流式细胞术分析表明，该化合物的细胞毒性作用与caspase-3激活有关。25 μ M的阿焦烯改变了B16 F10上α 4 β 1整合素的表达，并诱导这些细胞与内皮细胞单层的粘附显著减少。
In this study we evaluated the hypothesis that the antitumor activity of ajoene could be associated with its apoptosis-inducing effect, and with its ability to block the expression of the alpha(4)beta(1) integrin, in the murine melanoma B16F10 cells. Ajoene induced a significant reduction in B16F10 viability (IC(50)=62 microM), in a dose-dependent manner. Flow cytometric analysis showed that the cytotoxic effect of this compound was associated with caspase-3 activation. Ajoene at 25 microM altered the alpha(4)beta(1) integrin expression on B16F10, and induced a significant reduction in the adhesion of these cells to an endothelial cell monolayer.