Identification of Cell Surface Antigen Expression in Canine Hepatocellular Carcinoma Cell Lines
Identification of Cell Surface Antigen Expression in Canine Hepatocellular Carcinoma Cell Lines
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DOI：
10.1292/jvms.12-0549
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发表时间：
2013-06-01
影响因子：
1.2
通讯作者：
Tsuchiya, Ryo
中科院分区：
文献类型：
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作者：
Fujimoto, Ayumi;Neo, Sakurako;Tsuchiya, Ryo
犬肝细胞癌(CHCC)的表面抗原特征尚未阐明。本研究的目的是研究CHCC细胞系中被认为是干/祖细胞或癌细胞标志物的表面抗原。用流式细胞术检测CD29、CD34、CD44、CD90、CD133和DLK-1等多种抗原在四种CHCC细胞系中的表达。CD44、CD133和DLK-1在所有细胞株中均有表达，其中3株细胞表达CD29。这些结果表明，CD29、CD44、CD133和DLK-1有可能成为鉴定肝细胞癌的合适标记物，提示这些发现将有助于建立一种早期诊断手段来鉴定肝细胞的成熟过程。
The characteristics of surface antigens in canine hepatocellular carcinoma (cHCC) have not been clarified. The objective of this study was to investigate surface antigens, which are considered as stem/progenitor or cancer cell markers, in cHCC cell lines. Expression of various antigens including CD29, CD34, CD44, CD90, CD 133 and Dlk-1 was assessed in four cHCC cell lines by flow cytometry. CD44, CD133 and Dlk-1 expression was detectable in all cell lines, and three cell lines expressed CD29. These results indicate that CD29, CD44, CD 133 and Dlk-1 have potential as suitable markers in cHCC identification, suggesting that these findings will contribute to the establishment of an early diagnostic tool for the identification of hepatocellular maturation processes.