DNA methylation changes in normal liver tissues and hepatocellular carcinoma with different viral infection.
DNA methylation changes in normal liver tissues and hepatocellular carcinoma with different viral infection.
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DOI：
10.1016/j.yexmp.2010.01.002
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发表时间：
2010-04
影响因子：
3.6
通讯作者：
Kiviat, Nancy B.
中科院分区：
文献类型：
--
作者：
Feng, Qinghua;Stern, Joshua E.;Hawes, Stephen E.;Lu, Hiep;Jiang, Mingjun;Kiviat, Nancy B.
众所周知，肝细胞癌与乙肝病毒、丙型肝炎病毒和HVC有关。虽然表观遗传学改变已被报道与肝细胞癌有关，但HBc相关性肝细胞癌与丙型肝炎相关性肝细胞癌的表观遗传学特征是否不同尚不清楚。我们对65个存档肝组织块进行了甲基化检测，分析了10个基因(APC、CCND2、CDKN2A、GSTP1、HOXA9、RARB、RASSF1、RUNX、SFRP1和Twist1)的甲基化情况。有三个基因(APC、CCND2和GSTP1)在正常肝组织中频繁甲基化。5个基因(APC、CDKN2A、HOXA9、RASSF1和RUNX)在恶性肝组织中的甲基化频率显著高于正常肝组织。在肝细胞癌中，HOXA9、RASSF1和SFRP1的甲基化频率在乙肝病毒阳性的肝细胞癌中较高，而CDKN2A在丙型肝炎病毒阳性的肝细胞癌中的甲基化频率较高。我们的数据支持这样一种假设，即不同的病毒病因导致的肝癌与不同的表观遗传学变化有关。
Hepatocellular carcinoma (HCC) is known to be associated with both HBV and HCV and HVC. While epigenetic changes have been previously reported to be associated with hepatocellular carcinoma (HCC), whether the epigenetic profile of HBC associated HCC differs from that of HCV associated HCC is unclear. We analyzed DNA methylation of ten genes (APC, CCND2, CDKN2A, GSTP1, HOXA9, RARB, RASSF1, RUNX, SFRP1, and TWIST1) using MethyLight assays on 65 archived liver tissue blocks. Three genes (APC, CCND2, and GSTP1) were frequently methylated in normal liver tissues. Five genes (APC, CDKN2A, HOXA9, RASSF1, and RUNX) were significantly more frequently methylated in malignant liver tissues than normal liver tissues. Among HCC cases, HOXA9, RASSF1 and SFRP1 were methylated more frequently in HBV positive HCC cases, while CDKN2A were significantly more frequently methylated in HCV positive HCC cases. Our data support the hypothesis that HCC resulting from different viral etiologies are associated with different epigenetic changes.
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